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Literature summary for 1.6.2.2 extracted from
- Purification and properties of a membrane-bound NADH-cytochrome-b5 reductase from erythrocytes of the sipunculid worm, Phascolopsis gouldii (1989), Biochim. Biophys. Acta, 999, 147-156.
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol is essential for stability | Phascolopsis gouldii |
| EDTA is essential for stability | Phascolopsis gouldii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by NAD+ and ferrocyanide | Phascolopsis gouldii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
cytochrome b5 | - |
Phascolopsis gouldii | |
| 0.06 | - |
NADH | - |
Phascolopsis gouldii |  |
| 0.09 | - |
ferricyanide | - |
Phascolopsis gouldii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Phascolopsis gouldii | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Phascolopsis gouldii |
| 200000 | - |
oligomeric aggregate of detergent-solubilized enzyme in aqueous media, gel filtration | Phascolopsis gouldii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phascolopsis gouldii | - |
sipuncilid worm | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| O2, solubilized enzyme very sensitive to atmospheric oxygen | Phascolopsis gouldii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phascolopsis gouldii |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | soluble form | Phascolopsis gouldii | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
low MW aggregate, nitro blue tetrazolium as electron acceptor | Phascolopsis gouldii |
| 10.6 | - |
low MW aggregate, cytochrome b as electron acceptor | Phascolopsis gouldii |
| 138 | - |
low MW aggregate, dichlorophenolindophenol as electron acceptor | Phascolopsis gouldii |
| 230.5 | - |
high MW aggregate | Phascolopsis gouldii |
| 611.1 | - |
low MW aggregate, ferricyanide as electron acceptor | Phascolopsis gouldii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| liquid N2 | Phascolopsis gouldii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methemerythrin + NADH | - |
Phascolopsis gouldii | deoxymethemerythrin + NAD+ | - |
? | |
| NADH + ferricyanide | - |
Phascolopsis gouldii | NAD+ + H+ + ferrocyanide | - |
r | |
| NADH + ferricytochrome b5 | - |
Phascolopsis gouldii | NAD+ + H+ + ferrocytochrome b5 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Phascolopsis gouldii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
acceptor ferricyanide | Phascolopsis gouldii |
| 6 | 8 | acceptor Phascolopsis gouldii cytochrome b5 | Phascolopsis gouldii |
| 8 | - |
acceptor dichlorphenolindophenol | Phascolopsis gouldii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Phascolopsis gouldii | |
| NADH | - |
Phascolopsis gouldii | |
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