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Literature summary for 1.5.99.12 extracted from

  • Yuldashev, R.; Avalbaev, A.; Bezrukova, M.; Vysotskaya, L.; Khripach, V.; Shakirova, F.
    Cytokinin oxidase is involved in the regulation of cytokinin content by 24-epibrassinolide in wheat seedlings (2012), Plant Physiol. Biochem., 55, 1-6.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
semi-quantitative RT-PCR expression analysis Triticum aestivum

Inhibitors

Inhibitors Comment Organism Structure
24-epibrassinolide inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots Triticum aestivum

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
-
-
Triticum aestivum Bashkirskaya 24
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Triticum aestivum
-
seedling
-
Triticum aestivum
-
shoot
-
Triticum aestivum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isopentenyladenosine + acceptor + H2O
-
Triticum aestivum ? + reduced acceptor
-
?
isopentenyladenosine + acceptor + H2O
-
Triticum aestivum Bashkirskaya 24 ? + reduced acceptor
-
?

Synonyms

Synonyms Comment Organism
CKX
-
Triticum aestivum
cytokinin oxidase/dehydrogenase
-
Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Triticum aestivum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
assay at Triticum aestivum

Expression

Organism Comment Expression
Triticum aestivum 24-epibrassinolide inhibits both cytokinin oxidase activity and expression. Treatment with 0.4 mM 24-epibrassinolide leads to elevated cytokinin levels in roots and shoots, that are maintained only in the presence of 24-epibrassinolide, while the hormone removal leads to return of cytokinin concentration to the control level initially in the roots and then in the shoots down

General Information

General Information Comment Organism
malfunction inhibition of the enzyme by 24-epibrassinolide leads to activation of production of cytokinins in roots and shoots, of which excessive amounts are transferred into the storage forms Triticum aestivum
metabolism comparison of the functional spectra of cytokinins and brassinosteroids, overview Triticum aestivum
physiological function cytokinin oxidase is involved in the regulation of cytokinin content by 24-epibrassinolide in wheat seedlings Triticum aestivum