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Literature summary for 1.5.99.12 extracted from

  • Frebortova, J.; Novak, O.; Frebort, I.; Jorda, R.
    Degradation of cytokinins by maize cytokinin dehydrogenase is mediated by free radicals generated by enzymatic oxidation of natural benzoxazinones (2010), Plant J., 61, 467-481.
    View publication on PubMed
Search for more literature for 1.5.99.12

Activating Compound

Activating Compound Comment Organism Structure
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0041
-
 4-nitrosoresorcinol-1-monomethyl ether pH 5.0, in the presence of peroxidase, temperature not specified in the publication Zea mays
0.0062
-
 4-nitrosoresorcinol-1-monomethyl ether pH 5.0, in the presence of laccase, temperature not specified in the publication Zea mays
0.0089
-
 hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one pH 5.0, in the presence of peroxidase, temperature not specified in the publication Zea mays
0.0109
-
 hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one pH 5.0, in the presence of laccase, temperature not specified in the publication Zea mays
0.0113
-
 4-nitrosoresorcinol-1-monomethyl ether pH 7.0, in the presence of peroxidase, temperature not specified in the publication Zea mays
0.0345
-
 hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one pH 7.0, in the presence of laccase, temperature not specified in the publication Zea mays
0.067
-
 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical pH 5.0, temperature not specified in the publication Zea mays
0.113
-
 hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one pH 7.0, in the presence of peroxidase, temperature not specified in the publication Zea mays
0.192
-
 4-nitrosoresorcinol-1-monomethyl ether pH 5.0, temperature not specified in the publication Zea mays
0.25
-
 hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one Km above 0.25 mM, pH 7.0, temperature not specified in the publication Zea mays

Organism

Organism UniProt Comment Textmining
Zea mays
-
 variety Cellux 225
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information laccase and peroxidase catalyze oxidative cleavage of hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one (DIMBOA) to 4-nitrosoresorcinol-1-monomethyl ether (coniferron) which serves as a weak electron acceptor of CKX1. The oxidation of DIMBOA and coniferron generates transitional free radicals that are used by CKX1 as effective electron acceptors. hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-glucoside is not active as an electron acceptor for isozyme CKX1 Zea mays ?
-
 ?
N6-(2-isopentenyl)adenine + 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical + H2O
-
 Zea mays adenine + 3-methylbut-2-enal + ?
-
 ?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O 4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX Zea mays adenine + 3-methylbut-2-enal + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
 ?
N6-(2-isopentenyl)adenine + 4-nitrosoresorcinol-1-monomethyl ether + H2O 4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX Zea mays adenine + 3-methylbut-2-enal + reduced 4-nitrosoresorcinol-1-monomethyl ether
-
 ?
N6-(2-isopentenyl)adenine + hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one + H2O CKX1 is able to use hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one as a poor electron acceptor at neutral pH levels, but not in acidic pH levels Zea mays adenine + 3-methylbut-2-enal + reduced hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one
-
 ?

Synonyms

Synonyms Comment Organism
CKX1
-
 Zea mays
cytokinin dehydrogenase
-
 Zea mays