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Literature summary for 1.5.99.12 extracted from

  • Galuszka, P.; Frebortova, J.; Werner, T.; Yamada, M.; Strnad, M.; Schmulling, T.; Frebort, I.
    Cytokinin oxidase/dehydrogenase genes in barley and wheat: cloning and heterologous expression (2004), Eur. J. Biochem., 271, 3990-4002.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information isozymes TaCKX5a/b, TaCKX6a, and TaCKX2a expression are induced by pathogen infection, i.e. by Fusarium sp. or Puccinia sp. Triticum aestivum

Cloned(Commentary)

Cloned (Comment) Organism
gene HvCKX1, DNA sequence determination and analysis Hordeum vulgare
gene HvCKX2, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system Hordeum vulgare
gene HvCKX3, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae, transformation and overexpression in Arabidopsis thaliana and Nicotiana tabacum using the Agrobacterium tumefaciens strain GUS3101 system Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information overexpression of HvCKX3 in transgenic tobacco plants does not lead to increased enzyme activity or an altered phenotype Hordeum vulgare
additional information transgenic tobacco plants overexpressing HvCKX2 show a very strong phenotype of cytokinin deficiency with shorter interndes, highly increased formation of roots, and a dwarf growth habit, and died without producing seeds Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Hordeum vulgare 5739
-
vacuole
-
Hordeum vulgare 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58100
-
x * 58100, HvCKX3, amino acid sequence calculation Hordeum vulgare
58800
-
x * 58800, HvCKX2, amino acid sequence calculation Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
cv. Luxor, gene HvCKX7, genomic DNA, barley contains at least 7 different gene homologues, gene HvCKX7, genomic DNA
-
Hordeum vulgare Q84U27 cv. Luxor, gene HvCKX3, barley contains at least 7 different gene homologues
-
Hordeum vulgare Q8H6F6 cv. Luxor, gene HvCKX2, barley contains at least 7 different gene homologues
-
Hordeum vulgare Q94KI4 cv. Luxor, gene HvCKX1, barley contains at least 7 different gene homologues
-
Triticum aestivum Q94KI5 cv. Samantha, gene TaCKX1, wheat contains at least 8 different gene homologues
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme amino acid sequence contains 5 potential glycosylation sites Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
Hordeum vulgare
-
caryopsis
-
Hordeum vulgare
-
kernel
-
Hordeum vulgare
-
kernel developing, TaCKX6a Triticum aestivum
-
leaf young Hordeum vulgare
-
leaf young and adult Hordeum vulgare
-
leaf Puccinia-infected: TaCKX2a Triticum aestivum
-
additional information tissue specific and developmental expression of barley isozymes, overview Hordeum vulgare
-
additional information tissue specific expression of wheat isozymes Triticum aestivum
-
root young, TaCKX7a Triticum aestivum
-
seed TaCKX1, TaCKX2b, developing, TaCKX6a/b Triticum aestivum
-
seedling
-
Hordeum vulgare
-
seedling TaCKX1 in drought-stressed seedlings, TaCKX3 Triticum aestivum
-
spike Fusarium-infected: TaCKX5a/b and TaCKX6a Triticum aestivum
-
spikelet at early flowering: TaCKX7b and TaCKX8, at late flowering: TaCKX4 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytokinin + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
Hordeum vulgare ? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
?
cytokinin riboside + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
Hordeum vulgare ? + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
?
isopentenyladenine + 2,6-dichlorophenolindophenol
-
Hordeum vulgare adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
isopentenyladenine 9-glucoside + 2,6-dichlorophenolindophenol
-
Hordeum vulgare adenine 9-glucoside + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
isopentenyladenosine + 2,6-dichlorophenolindophenol
-
Hordeum vulgare adenosine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
isopentenyladenosine monophosphate + 2,6-dichlorophenolindophenol
-
Hordeum vulgare adenosine monophosphate + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
additional information tissue and isozyme-specific substrate specificity Hordeum vulgare ?
-
?
additional information tissue and isozyme-specific substrate specificity, recombinant HvCKX2 prefers cytokinin ribosides as substrates Hordeum vulgare ?
-
?
trans-zeatin + 2,6-dichlorophenolindophenol cis-zeatin is utilized only by some tissue-specific forms but not by HvCKX2 Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?
trans-zeatin O-glucoside + 2,6-dichlorophenolindophenol
-
Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?
trans-zeatin riboside + 2,6-dichlorophenolindophenol
-
Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?
trans-zeatin riboside O-glucoside + 2,6-dichlorophenolindophenol
-
Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?
trans-zeatin ribotide + 2,6-dichlorophenolindophenol
-
Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?
trans-zeatin-9-glucoside + 2,6-dichlorophenolindophenol
-
Hordeum vulgare ? + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

Subunits Comment Organism
? x * 58100, HvCKX3, amino acid sequence calculation Hordeum vulgare
? x * 58800, HvCKX2, amino acid sequence calculation Hordeum vulgare

Synonyms

Synonyms Comment Organism
CKX
-
Hordeum vulgare
cytokinin oxidase/dehydrogenase
-
Hordeum vulgare
cytokinin oxidase/dehydrogenase
-
Triticum aestivum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 Hordeum vulgare
4.5
-
recombinant HvCKX2 expressed in tobacco, native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 Hordeum vulgare
7 7.5 native overall enzyme activity in grains and leaves shows 2 maxima at pH 4.5 and at pH 7.0-7.5 Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
FAD binding motif Hordeum vulgare
FAD binding motif Triticum aestivum

pI Value

Organism Comment pI Value Maximum pI Value
Hordeum vulgare sequence calculation
-
6
Hordeum vulgare sequence calculation
-
7.1