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Literature summary for 1.5.99.12 extracted from

  • Frebortova, J.; Fraaije, M.W.; Galuszka, P.; Sebela, M.; Pec, P.; Hrbac, J.; Novak, O.; Bilyeu, K.D.; English, J.T.; Frebort, I.
    Catalytic reaction of cytokinin dehydrogenase: preference for quinones as electron acceptors (2004), Biochem. J., 380, 121-130.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of ZmCKX1 in Pichia pastoris Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics, redox potential of electron acceptors Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(2-isopentenyl)adenine + FAD Zea mays the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine adenine + 3-methylbut-2-enal + FADH2
-
?

Organism

Organism UniProt Comment Textmining
Zea mays Q9T0N8 enzyme and gene ZmCKX1
-

Reaction

Reaction Comment Organism Reaction ID
N6-dimethylallyladenine + acceptor + H2O = adenine + 3-methylbut-2-enal + reduced acceptor reaction scheme and mechanism, reduction of N6-(2-isopentenyl)adenine is rapidly performed, the reaction with O2 is slowly Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity with cytokinins, overiew Zea mays ?
-
?
N6-(2-isopentenyl)adenine + 1,4-naphthoquinone
-
Zea mays adenine + 3-methylbut-2-enal + reduced 1,4-naphthoquinone
-
?
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone i.e. coenzyme Q0 Zea mays adenine + 3-methylbut-2-enal + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
?
N6-(2-isopentenyl)adenine + 2,6-dichlorophenolindophenol
-
Zea mays adenine + 3-methylbut-2-enal + reduced 2,6-dichlorophenolindophenol
-
?
N6-(2-isopentenyl)adenine + caffeic acid
-
Zea mays adenine + 3-methylbut-2-enal + ?
-
?
N6-(2-isopentenyl)adenine + coenzyme Q1
-
Zea mays adenine + 3-methylbut-2-enal + reduced coenzyme Q1
-
?
N6-(2-isopentenyl)adenine + cytochrome c
-
Zea mays adenine + 3-methylbut-2-enal + reduced cytochrome c
-
?
N6-(2-isopentenyl)adenine + daphnoretin
-
Zea mays adenine + 3-methylbut-2-enal + ?
-
?
N6-(2-isopentenyl)adenine + FAD
-
Zea mays adenine + 3-methylbut-2-enal + FADH2
-
?
N6-(2-isopentenyl)adenine + FAD the natural terminal electron acceptor probably is a p-quinone or a similar compound, high substrate specificity for N6-(2-isopentenyl)adenine Zea mays adenine + 3-methylbut-2-enal + FADH2
-
?
N6-(2-isopentenyl)adenine + o-coumaric acid
-
Zea mays adenine + 3-methylbut-2-enal + ?
-
?
N6-(2-isopentenyl)adenine + O2 oxidative degradation of cytokinins, slow reaction, low substrate specificity Zea mays adenine + 3-methylbut-2-enal + H2O2
-
?
N6-(2-isopentenyl)adenine + plantacyanine electron acceptor from spinach Zea mays adenine + 3-methylbut-2-enal + reduced plantacyanine
-
?
N6-(2-isopentenyl)adenine + rosmarinic acid
-
Zea mays adenine + 3-methylbut-2-enal + ?
-
?
N6-(2-isopentenyl)adenine + scopoletin
-
Zea mays adenine + 3-methylbut-2-enal + ?
-
?
N6-(2-isopentenyl)adenine + spinach plantacyanine
-
Zea mays adenine + 3-methylbut-2-enal + reduced spinach plantacyanine
-
r
N6-(2-isopentenyl)adenine + vitamin K1
-
Zea mays adenine + 3-methylbut-2-enal + reduced vitamin K1
-
?
N6-(2-isopentenyl)adenine + vitamin K3
-
Zea mays adenine + 3-methylbut-2-enal + reduced vitamin K3
-
?
N6-(2-isopentenyl)adenosine + FAD
-
Zea mays adenosine + 3-methylbut-2-enal + FADH2
-
?
zeatin + FAD
-
Zea mays ? + FADH2
-
?

Synonyms

Synonyms Comment Organism
CKX
-
Zea mays
cytokinin oxidase/dehydrogenase
-
Zea mays
More enzyme belongs to the plant CKX family Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information at pH above pH 6.5 polymerization of the quinone acceptors occur Zea mays
0.1
-
O2 below, pH 8.0, recombinant enzyme Zea mays
0.3
-
caffeic acid pH 6.5, recombinant enzyme Zea mays
0.3
-
o-coumaric acid pH 6.5, recombinant enzyme Zea mays
0.4
-
rosmarinic acid pH 6.5, recombinant enzyme Zea mays
0.6
-
O2 pH 6.5, recombinant enzyme Zea mays
0.8
-
cytochrome c pH 6.5, recombinant enzyme Zea mays
0.9
-
spinach plantacyanine pH 6.5, recombinant enzyme Zea mays
1
-
daphnoretin pH 6.5, recombinant enzyme Zea mays
1.4
-
scopoletin pH 6.5, recombinant enzyme Zea mays
2.3
-
vitamin K1 pH 6.5, recombinant enzyme Zea mays
85.4
-
vitamin K3 pH 6.5, recombinant enzyme Zea mays
123.1
-
coenzyme Q1 pH 6.5, recombinant enzyme Zea mays
129.8
-
N6-(2-isopentenyl)adenosine pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone Zea mays
143.6
-
2,3-dimethoxy-5-methyl-1,4-benzoquinone pH 6.5, recombinant enzyme Zea mays
143.6
-
N6-(2-isopentenyl)adenine pH 6.5, recombinant enzyme, with cofactor 2,3-dimethoxy-5-methyl-1,4-benzoquinone Zea mays
169.6
-
1,4-Naphthoquinone pH 6.5, recombinant enzyme Zea mays
178.4
-
2,6-dichlorophenolindophenol pH 6.5, recombinant enzyme Zea mays
278
-
2,6-dichlorophenolindophenol pH 8.0, recombinant enzyme Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
substrate-dependent pH-profile, overview Zea mays

Cofactor

Cofactor Comment Organism Structure
FAD covalently bound, redox potential analysis Zea mays
additional information enzyme shows a preference for quinones as electron acceptors Zea mays