Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43 cells | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
T525A | the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials | Cereibacter sphaeroides |
Y501F | the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials | Cereibacter sphaeroides |
Y501F/T525A | the mutation decreases the midpoint potentials of the iron-sulfur cluster resulting in a decrease in steady-state ubiquinone reductase activity and in electron transfer flavoprotein semiquinone disproportionation, there is no detectable effect of the mutation on the flavin midpoint potentials | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
SDS-PAGE | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose colmn chromatography and Source 15Q column chromatography | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced electron-transferring flavoprotein + ubiquinone-2 | - |
Cereibacter sphaeroides | electron-transferring flavoprotein + ubiquinol-2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
electron transfer flavoprotein-ubiquinone oxidoreductase | - |
Cereibacter sphaeroides |
ETF-QO | - |
Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | iron-sulfur flavoprotein that contains a single [4Fe-4S]2+,1+ | Cereibacter sphaeroides | |
FAD | contains one FAD | Cereibacter sphaeroides |