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Literature summary for 1.5.1.6 extracted from

  • Krupenko, S.A.; Wagner, C.; Cook, R.J.
    Cysteine 707 is involved in the dehydrogenase active site of rat 10-formyltetrahydrofolate dehydrogenase (1995), J. Biol. Chem., 270, 519-522.
    View publication on PubMed
Search for more literature for 1.5.1.6

Cloned(Commentary)

Cloned (Comment) Organism
expression of FDH cDNA, subcloned in the pVL 1393 baculovirus vector, in Sf9 insect cells, using the MaxBac expression system Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C707A mutant with a complete loss of dehydrogenase activity of 10-formyltetrahydrofolate and propanal, but hydrolase activity and binding of NADP+ is unchanged Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99000
-
 4 * 99000, wild-type FDH and C707A mutant Rattus norvegicus
393200
-
 C707A mutant FDH, molecular size detector employing laser-light scattering methodology Rattus norvegicus
398800
-
 wild-type FDH, molecular size detector employing laser-light scattering methodology Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of a C707A mutant of FDH Rattus norvegicus
purification of recombinant liver FDH Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyl-5,8-dideazafolate + NADP+ + H2O
-
 Rattus norvegicus 5,8-dideazafolate + CO2 + NADPH + H+
-
 ?
10-formyltetrahydrofolate + NADP+ + H2O Cys-707 is a key residue of the dehydrogenase active site and acts as nucleophile in the formation of an enzyme-linked thiohemiacetal intermediate Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
additional information enzyme exhibit additional to 10-formyltetrahydrofolate dehydrogenase/hydrolase activities NADP+-dependent aldehyde dehydrogenase activity with propanal as preferred substrate Rattus norvegicus ?
-
 ?
additional information 10-formyltetrahydrofolate dehydrogenase/hydrolase activities occur at the same time and are associated with separate active sites Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 99000, wild-type FDH and C707A mutant Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
NADP+ NADP+-dependent Rattus norvegicus
tetrahydrofolate
-
 Rattus norvegicus