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Literature summary for 1.5.1.6 extracted from

  • Scrutton, M.C.; Beis, I.
    Inhibitory effects of histidine and their reversal. The roles of pyruvate carboxylase and N10-formyltetrahydrofolate dehydrogenase (1979), Biochem. J., 177, 833-846.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information not activated by methionine or ethionine Rattus norvegicus
NADPH activates at concentrations and in ratio with NADP+ consistent with those in rat liver in vivo, activation is associated with higher Ki-value for tetrahydrofolate Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by NADPH Rattus norvegicus
tetrahydrofolate competitive to 10-formyltetrahydrofolate and non-competitive to NADP+; very strong product inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00092
-
NADP+ at pH 7 Rattus norvegicus
0.0045
-
10-formyltetrahydrofolate at pH 7 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
108000
-
4 * 108000, SDS-PAGE Rattus norvegicus
413000
-
gel filtration, sedimentation analysis Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
250fold purification Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ sequential mechanism of substrate addition Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
kidney less activity Rattus norvegicus
-
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7 0.8 at 25°C Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, 0.05 M phosphate buffer, pH 7.2, 50%-saturated (NH4)2SO4, 20% glycerol, 1 mM EDTA, 0.1 mM dithioerythritol, 0.01 mM phenylmethanesulfonyl fluoride, several months, stable Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
?
additional information hydrolysis catalyzed by enzyme at 21% of the rate of CO2 formation Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 108000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
enzyme activity at 37°C is twice that at 25°C Rattus norvegicus
37
-
maximal catalytic capacity at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
NADP+ specific for Rattus norvegicus
NADP+ NADP+-dependent Rattus norvegicus
tetrahydrofolate
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
tetrahydrofolate (-)-isomer Rattus norvegicus