Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by methionine or ethionine | Rattus norvegicus | |
NADPH | activates at concentrations and in ratio with NADP+ consistent with those in rat liver in vivo, activation is associated with higher Ki-value for tetrahydrofolate | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by NADPH | Rattus norvegicus | |
tetrahydrofolate | competitive to 10-formyltetrahydrofolate and non-competitive to NADP+; very strong product inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00092 | - |
NADP+ | at pH 7 | Rattus norvegicus | |
0.0045 | - |
10-formyltetrahydrofolate | at pH 7 | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
4 * 108000, SDS-PAGE | Rattus norvegicus |
413000 | - |
gel filtration, sedimentation analysis | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
250fold purification | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O = tetrahydrofolate + CO2 + NADPH + H+ | sequential mechanism of substrate addition | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | less activity | Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.7 | 0.8 | at 25°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-70°C, 0.05 M phosphate buffer, pH 7.2, 50%-saturated (NH4)2SO4, 20% glycerol, 1 mM EDTA, 0.1 mM dithioerythritol, 0.01 mM phenylmethanesulfonyl fluoride, several months, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Rattus norvegicus | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
additional information | hydrolysis catalyzed by enzyme at 21% of the rate of CO2 formation | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 108000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activity at 37°C is twice that at 25°C | Rattus norvegicus |
37 | - |
maximal catalytic capacity at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Rattus norvegicus | |
NADP+ | specific for | Rattus norvegicus | |
NADP+ | NADP+-dependent | Rattus norvegicus | |
tetrahydrofolate | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
tetrahydrofolate | (-)-isomer | Rattus norvegicus |