Cloned (Comment) | Organism |
---|---|
expressed as a His-tagged fusion protein | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-4H-pyrimidin-5-carboxylic acid ethyl ester | both monastrol (R) and (S) enantiomers fit well in the ligand-binding pocket of LdPTR1. Monastrol is a potent inhibitor of PTR1 in Leishmania, it inhibits proliferation of amastigotes in macrophage cultures infected with an Leishmania donovani clinical isolate, with no host cytotoxicity | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biopterin + 2 NADPH + 2 H+ | - |
Leishmania donovani | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LdPTR1 | - |
Leishmania donovani |
pteridine reductase 1 | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000428 | - |
4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-4H-pyrimidin-5-carboxylic acid ethyl ester | pH 4.8, 30°C | Leishmania donovani |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
pH 4.8, 30°C | Leishmania donovani | 4-(3-hydroxyphenyl)-6-methyl-2-thioxo-1,2,3,4-tetrahydro-4H-pyrimidin-5-carboxylic acid ethyl ester |