Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.33 extracted from

  • Leblanc, E.; Papadopoulou, B.; Bernatchez, C.; Ouellette, M.
    Residues involved in co-factor and substrate binding of the short-chain dehydrogenase/reductase PTR1 producing methotrexate resistance in Leishmania (1998), Eur. J. Biochem., 251, 768-774.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Leishmania tarentolae

Protein Variants

Protein Variants Comment Organism
K199E The mutant enzymes Y38D, Y195F, Y195W, and K199R are inactive even if they are purified as tetramers Leishmania tarentolae
Y175D mutant enzyme Y175D shows properties similar to wild type enzyme. The mutant enzymes Y38D, Y195F, Y195W, and K199R are inactive even if they are purified as tetramers Leishmania tarentolae
Y195F The mutant enzymes Y38D, Y195F, Y195W, and K199R are inactive even if they are purified as tetramers Leishmania tarentolae
Y195W The mutant enzymes Y38D, Y195F, Y195W, and K199R are inactive even if they are purified as tetramers Leishmania tarentolae
Y38D The mutant enzymes Y38D, Y195F, Y195W, and K199R are inactive even if they are purified as tetramers Leishmania tarentolae

Inhibitors

Inhibitors Comment Organism Structure
additional information
-
Leishmania tarentolae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0032
-
biopterin mutant enzyme Y175F Leishmania tarentolae
0.0035
-
biopterin wild type enzyme Leishmania tarentolae

Organism

Organism UniProt Comment Textmining
Leishmania tarentolae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH
-
Leishmania tarentolae 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
Dihydrobiopterin + NADPH
-
Leishmania tarentolae ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
biopterin mutant enzyme Y175F Leishmania tarentolae
0.23
-
biopterin wild type enzyme Leishmania tarentolae