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Literature summary for 1.5.1.33 extracted from

  • Nare, B.; Luba, J.; Hardy, L.W.; Beverley, S.
    New approaches to Leishmania chemotherapy: pteridine reductase 1 (PTR1) as a target and modulator of antifolate sensitivity (1997), Parasitology, 114, 101-110.
No PubMed abstract available
Search for more literature for 1.5.1.33

Application

Application Comment Organism
pharmacology successful antifolate chemotherapy in Leishmania will have to target simultaneously both pterine reductase 1 and dihydrofolate reductase-thymidylate synthase Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
dihydrobiopterin substrate inhibition Leishmania major
dihydrofolate substrate inhibition Leishmania major
additional information
-
 Leishmania major

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NADPH NADPH Leishmania major

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 4 * 30000 Leishmania major

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Reaction

Reaction Comment Organism Reaction ID
5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ ordered sequential reaction mechanism Leishmania major
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH
-
 Leishmania major 5,6,7,8-Tetrahydrobiopterin + NADP+
-
 ?
Dihydrobiopterin + NADPH
-
 Leishmania major ?
-
 ?
Dihydrofolate + NADPH
-
 Leishmania major 5,6,7,8-Tetrahydrofolate + NADP+
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 30000 Leishmania major

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 biopterin as substrate Leishmania major
6
-
 folate as substrate Leishmania major