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Literature summary for 1.5.1.3 extracted from

  • Czekster, C.M.; Vandemeulebroucke, A.; Blanchard, J.S.
    Kinetic and chemical mechanism of the dihydrofolate reductase from Mycobacterium tuberculosis (2011), Biochemistry, 50, 367-375.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
5,6,7,8-tetrahydrofolate non-competitive product inhibition Mycobacterium tuberculosis
NADP+ non-competitive product inhibition Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
NADPH Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
0.0016
-
7,8-dihydrofolate with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
0.067
-
NADPH at 25 °C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7,8-dihydrofolate + NADH + H+ Mycobacterium tuberculosis
-
5,6,7,8-tetrahydrofolate + NAD+
-
?
7,8-dihydrofolate + NADPH + H+ Mycobacterium tuberculosis
-
5,6,7,8-tetrahydrofolate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADH + H+
-
Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NAD+
-
?
7,8-dihydrofolate + NADPH + H+
-
Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NADP+
-
?

Synonyms

Synonyms Comment Organism
DHFR
-
Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6
-
7,8-dihydrofolate with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis
2.3
-
7,8-dihydrofolate with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Mycobacterium tuberculosis
NADPH
-
Mycobacterium tuberculosis