Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydrofolate | non-competitive product inhibition | Mycobacterium tuberculosis | |
NADP+ | non-competitive product inhibition | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
NADPH | Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
0.0016 | - |
7,8-dihydrofolate | with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
0.067 | - |
NADPH | at 25 °C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADH + H+ | Mycobacterium tuberculosis | - |
5,6,7,8-tetrahydrofolate + NAD+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | Mycobacterium tuberculosis | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADH + H+ | - |
Mycobacterium tuberculosis | 5,6,7,8-tetrahydrofolate + NAD+ | - |
? | |
7,8-dihydrofolate + NADPH + H+ | - |
Mycobacterium tuberculosis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHFR | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
7,8-dihydrofolate | with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis | |
2.3 | - |
7,8-dihydrofolate | with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5 | Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycobacterium tuberculosis | |
NADPH | - |
Mycobacterium tuberculosis |