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Literature summary for 1.5.1.3 extracted from

  • Xu, Y.; Feller, G.; Gerday, C.; Glansdorff, N.
    Moritella cold-active dihydrofolate reductase: are there natural limits to optimization of catalytic efficiency at low temperature? (2003), J. Bacteriol., 185, 5519-5526.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Moritella profunda

Inhibitors

Inhibitors Comment Organism Structure
methotrexate IC50: 14.5 nM Moritella profunda
trimethoprim IC50: 0.0048 mM Moritella profunda

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
NADPH pH 7.0, 30°C Moritella profunda
0.0099
-
NADPH pH 7.0, 5°C Moritella profunda
0.0123
-
NADPH pH 7.0, 20°C Moritella profunda
16.4
-
7,8-dihydrofolate pH 7.0, 20°C Moritella profunda
19.8
-
7,8-dihydrofolate pH 7.0, 30°C Moritella profunda
21.7
-
7,8-dihydrofolate pH 7.0, 5°C Moritella profunda

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18290
-
mass spectrometry Moritella profunda
19000
-
gel filtration Moritella profunda

Organism

Organism UniProt Comment Textmining
Moritella profunda Q70YQ6 sp. nov.
-

Purification (Commentary)

Purification (Comment) Organism
-
Moritella profunda

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Moritella profunda 5,6,7,8-tetrahydrofolate + NADP+
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
34
-
-
Moritella profunda

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 65% of maximal activity, 50°C: about 70% of maximal activity Moritella profunda

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
glycerol and 2-mercaptoethanol shift the midpoint of the thermal transition upwards by about 25°C Moritella profunda
-
-
cold denaturation below Moritella profunda
35
-
15 min, stable Moritella profunda
45
-
half-life: 25 min Moritella profunda
50
-
half-life: 18 min in presence of 0.5 mM 2-mercaptoethanol and 0.5% glycerol Moritella profunda

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Moritella profunda

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Moritella profunda

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000145
-
IC50: 14.5 nM Moritella profunda methotrexate
0.0048
-
IC50: 0.0048 mM Moritella profunda trimethoprim