Cloned (Comment) | Organism |
---|---|
- |
Moritella profunda |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | IC50: 14.5 nM | Moritella profunda | |
trimethoprim | IC50: 0.0048 mM | Moritella profunda |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0096 | - |
NADPH | pH 7.0, 30°C | Moritella profunda | |
0.0099 | - |
NADPH | pH 7.0, 5°C | Moritella profunda | |
0.0123 | - |
NADPH | pH 7.0, 20°C | Moritella profunda | |
16.4 | - |
7,8-dihydrofolate | pH 7.0, 20°C | Moritella profunda | |
19.8 | - |
7,8-dihydrofolate | pH 7.0, 30°C | Moritella profunda | |
21.7 | - |
7,8-dihydrofolate | pH 7.0, 5°C | Moritella profunda |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18290 | - |
mass spectrometry | Moritella profunda |
19000 | - |
gel filtration | Moritella profunda |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moritella profunda | Q70YQ6 | sp. nov. | - |
Purification (Comment) | Organism |
---|---|
- |
Moritella profunda |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Moritella profunda | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
34 | - |
- |
Moritella profunda |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 65% of maximal activity, 50°C: about 70% of maximal activity | Moritella profunda |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
glycerol and 2-mercaptoethanol shift the midpoint of the thermal transition upwards by about 25°C | Moritella profunda |
- |
- |
cold denaturation below | Moritella profunda |
35 | - |
15 min, stable | Moritella profunda |
45 | - |
half-life: 25 min | Moritella profunda |
50 | - |
half-life: 18 min in presence of 0.5 mM 2-mercaptoethanol and 0.5% glycerol | Moritella profunda |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Moritella profunda |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Moritella profunda |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000145 | - |
IC50: 14.5 nM | Moritella profunda | methotrexate | |
0.0048 | - |
IC50: 0.0048 mM | Moritella profunda | trimethoprim |