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Literature summary for 1.5.1.3 extracted from

  • White, E.L.; Ross, L.J.; Cunningham, A.; Escuyer, V.
    Cloning, expression, and characterization of Mycobacterium tuberculosis dihydrofolate reductase (2004), FEMS Microbiol. Lett., 232, 101-105.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter Homo sapiens
expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
methotrexate IC50: 1.2 nM Homo sapiens
methotrexate IC50: 8.3 nM Mycobacterium tuberculosis
trimethoprim IC50: 0.0825 mM Homo sapiens
trimethoprim IC50: 0.0165 mM Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
7,8-dihydrofolate pH 7.3, 37°C Homo sapiens
0.0042
-
NADPH pH 7.3, 37°C Mycobacterium tuberculosis
0.0045
-
7,8-dihydrofolate pH 7.3, 37°C Mycobacterium tuberculosis
0.0091
-
NADPH pH 7.3, 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
KCl maximal activity at 0.5 M Homo sapiens
KCl steady increase in activity from 0 to 1 M KCl Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17643
-
1 * 17643, MALDI-TOF Homo sapiens
17643
-
1 * 17643, MALDI-TOF Mycobacterium tuberculosis
19000
-
gel filtration Homo sapiens
19000
-
gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.5
-
pH 7.0, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH
-
Mycobacterium tuberculosis 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH
-
Mycobacterium tuberculosis H37Rv 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 17643, MALDI-TOF Homo sapiens
monomer 1 * 17643, MALDI-TOF Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity at pH 9 is 8% of the activity at pH 5 Homo sapiens
5 9 activity at pH 9 is 8% of the activity at pH 5 Mycobacterium tuberculosis

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0000012
-
IC50: 1.2 nM Homo sapiens methotrexate
0.0000083
-
IC50: 8.3 nM Mycobacterium tuberculosis methotrexate
0.0165
-
IC50: 0.0165 mM Mycobacterium tuberculosis trimethoprim
0.0825
-
IC50: 0.0825 mM Homo sapiens trimethoprim