Cloned (Comment) | Organism |
---|---|
expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter | Homo sapiens |
expressed in inclusion bodies in high yield in Escherichia coli under the control of the T7 promoter | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | IC50: 1.2 nM | Homo sapiens | |
methotrexate | IC50: 8.3 nM | Mycobacterium tuberculosis | |
trimethoprim | IC50: 0.0825 mM | Homo sapiens | |
trimethoprim | IC50: 0.0165 mM | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
7,8-dihydrofolate | pH 7.3, 37°C | Homo sapiens | |
0.0042 | - |
NADPH | pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.0045 | - |
7,8-dihydrofolate | pH 7.3, 37°C | Mycobacterium tuberculosis | |
0.0091 | - |
NADPH | pH 7.3, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximal activity at 0.5 M | Homo sapiens | |
KCl | steady increase in activity from 0 to 1 M KCl | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17643 | - |
1 * 17643, MALDI-TOF | Homo sapiens |
17643 | - |
1 * 17643, MALDI-TOF | Mycobacterium tuberculosis |
19000 | - |
gel filtration | Homo sapiens |
19000 | - |
gel filtration | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
- |
Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.5 | - |
pH 7.0, 37°C | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Homo sapiens | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Mycobacterium tuberculosis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Mycobacterium tuberculosis H37Rv | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 17643, MALDI-TOF | Homo sapiens |
monomer | 1 * 17643, MALDI-TOF | Mycobacterium tuberculosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity at pH 9 is 8% of the activity at pH 5 | Homo sapiens |
5 | 9 | activity at pH 9 is 8% of the activity at pH 5 | Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000012 | - |
IC50: 1.2 nM | Homo sapiens | methotrexate | |
0.0000083 | - |
IC50: 8.3 nM | Mycobacterium tuberculosis | methotrexate | |
0.0165 | - |
IC50: 0.0165 mM | Mycobacterium tuberculosis | trimethoprim | |
0.0825 | - |
IC50: 0.0825 mM | Homo sapiens | trimethoprim |