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Literature summary for 1.5.1.3 extracted from

  • Cinquina, C.C.; Grogan, E.; Sun, R.; Lin, S.F.; Beardsley, G.P.; Miller, G.
    Dihydrofolate reductase from Kaposi's sarcoma-associated herpesvirus (2000), Virology, 268, 201-217.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Human gammaherpesvirus 8

Protein Variants

Protein Variants Comment Organism
additional information C-terminal-truncated mutant with lower activity Human gammaherpesvirus 8

Inhibitors

Inhibitors Comment Organism Structure
7,8-dihydrofolate substrate inhibition at concentration above 0.008 mM for native enzyme and at concentration above 0.001 mM for the recombinant enzyme Human gammaherpesvirus 8
aminopterin
-
Homo sapiens
aminopterin
-
Human gammaherpesvirus 8
methotrexate recombinant enzyme Homo sapiens
methotrexate
-
Human gammaherpesvirus 8
piritrexim recombinant enzyme Homo sapiens
piritrexim
-
Human gammaherpesvirus 8
pyrimethamine recombinant enzyme Homo sapiens
pyrimethamine
-
Human gammaherpesvirus 8
trimethoprim recombinant enzyme Homo sapiens
trimethoprim
-
Human gammaherpesvirus 8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00039
-
7,8-dihydrofolate recombinant enzyme Homo sapiens
0.001
-
NADPH for native and recombinant enzymes, monophasic Homo sapiens
0.001
-
NADPH for native and recombinant enzymes, monophasic Human gammaherpesvirus 8
0.0024
-
7,8-dihydrofolate native enzyme Human gammaherpesvirus 8

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus wild-type and recombinant viral enzyme in transfected cells Human gammaherpesvirus 8 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
methotrexate-resistant WIL-2 lymphoblastoid cells
-
Human gammaherpesvirus 8
-
human virus produced in cultured lymphoma derived B-cells
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Human gammaherpesvirus 8

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADPH
-
Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
?
7,8-dihydrofolate + NADPH
-
Human gammaherpesvirus 8 5,6,7,8-tetrahydrofolate + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7
-
7,8-dihydrofolate native enzyme Human gammaherpesvirus 8
16.8
-
7,8-dihydrofolate recombinant enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens
NADPH
-
Human gammaherpesvirus 8

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
additional information
-
additional information Ki-values for the native enzyme are higher than for the recombinant enzyme Human gammaherpesvirus 8
0.000000009
-
methotrexate recombinant enzyme Homo sapiens
0.0000002
-
methotrexate
-
Human gammaherpesvirus 8
0.00000061
-
aminopterin
-
Human gammaherpesvirus 8
0.000001
-
pyrimethamine recombinant enzyme Homo sapiens
0.0000039
-
piritrexim
-
Human gammaherpesvirus 8
0.000008
-
piritrexim recombinant enzyme Homo sapiens
0.000029
-
pyrimethamine
-
Human gammaherpesvirus 8
0.00081
-
trimethoprim recombinant enzyme Homo sapiens
0.0023
-
trimethoprim
-
Human gammaherpesvirus 8