Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | hDHFR-2, activation is maximal at 0.5 M | Haloferax volcanii |
Cloned (Comment) | Organism |
---|---|
overexpression of hdrB gene in Escherichia coli | Haloferax volcanii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of hdrAgene, hdrB gene and double deletion mutants | Haloferax volcanii |
General Stability | Organism |
---|---|
isozyme hDHFR-1, loss of more than 80% activity after exposure to 0.2 M KCl for 24 h at 24°C | Haloferax volcanii |
isozyme hDHFR-2 loss of less than 10% activity after exposure to 40 mM KCl for 18 h at 52°C | Haloferax volcanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
trimethoprim | hDHFR-1 is much more resistant than hDHFR-2 | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | - |
isozyme hDHFR-1 from gene hdrA and isozyme hDHFR-2 from gene hdrB | - |
Haloferax volcanii | - |
extremely halophilic archaebacterium | - |
Purification (Comment) | Organism |
---|---|
isozyme hDHFR-2 from hdrB gene overexpressed in Escherichia coli | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Haloferax volcanii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hDHFR-1 | - |
Haloferax volcanii |
hDHFR-2 | - |
Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
isozyme hDHFR-1 | Haloferax volcanii |
6 | - |
isozyme hDHFR-2 | Haloferax volcanii |