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Literature summary for 1.5.1.3 extracted from
- Cloning of the Escherichia coli K-12 dihydrofolate reductase gene following mu-mediated transposition (1980), Gene, 8, 255-265.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| folA gene, into multicopy vector pBR322, gene enrichment by Mu-mediated transposition | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Escherichia coli | |
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