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Literature summary for 1.5.1.3 extracted from
- Purification and characterization of beef liver dihydrofolate reductase (1976), Arch. Biochem. Biophys., 172, 289-300.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
1 * 21300, SDS-PAGE | Bos taurus |
| 22500 | - |
equilibrium sedimentation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 1 year | Bos taurus |
| 4°C, several weeks | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 21300, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Bos taurus |
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