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Literature summary for 1.5.1.3 extracted from
- Purification and immunochemical charaterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild-carrot cells (1987), EMBO J., 6, 1853-1858.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Daucus carota |  |
| aminopterin | - |
Daucus carota | |
| methotrexate | - |
Daucus carota | |
| N-ethylmaleimide | - |
Daucus carota | |
| N10-formylaminopterin | - |
Daucus carota | |
| p-hydroxymercuribenzoate | - |
Daucus carota | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, dihydrofolate reductase subunit, SDS-PAGE | Daucus carota |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
wild carrot, enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function | Daucus carota |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.8 | - |
purified enzyme | Daucus carota |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function, 286000 MW complex, 45000 MW dihydrofolate reductase subunit and 4 other polypeptide chains: 95000, 70000, 50000, 26000 | Daucus carota |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
assay at | Daucus carota |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay at pH 7.2, no activity at pH 5.9 | Daucus carota |
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Release 2023.1 (January 2023)
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