Application | Comment | Organism |
---|---|---|
medicine | enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer | Candida albicans |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Candida albicans | |
methotrexate | - |
Candida albicans | |
additional information | overview | Candida albicans | |
trimethoprim | - |
Candida albicans | |
Urea | - |
Candida albicans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Candida albicans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
recombinant from E. coli, gel filtration | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | Candida albicans | key enzyme in biosynthesis of purines, pyrimidines and several amino acids | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
additional information | Candida albicans | key enzyme in the biosynthesis of purines, pyrimidines and several amino acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Candida albicans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
250 | - |
purified enzyme | Candida albicans |
Storage Stability | Organism |
---|---|
4°C, above 1 mg/ml protein concentration, 1 month | Candida albicans |
4°C, pure, 20% glycerol, several weeks | Candida albicans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | key enzyme in biosynthesis of purines, pyrimidines and several amino acids | Candida albicans | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
additional information | - |
Candida albicans | ? | - |
? | |
additional information | key enzyme in the biosynthesis of purines, pyrimidines and several amino acids | Candida albicans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, recombinant from Escherichia coli, SDS-PAGE | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Candida albicans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000015 | - |
methotrexate | recombinant from E. coli | Candida albicans | |
0.0043 | - |
trimethoprim | recombinant from E. coli | Candida albicans |