KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18500 | - |
? * 18500, SDS-PAGE, isozyme I and II | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isoenzymes type I and type II, resistant to trimethoprim | - |
Purification (Comment) | Organism |
---|---|
trimethoprim-resistant | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.4 | - |
isozyme II | Escherichia coli |
85 | - |
isozyme I | Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
? | ? * 18500, SDS-PAGE, isozyme I and II | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
trimethoprim-resistant, form 2: no detectable pH-optimum, continuous increase of activity as pH decreases from pH 9.0 to pH 4.0 | Escherichia coli |
4.5 | - |
trimethoprim-resistant, 7,8-dihydrofolate, form I | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki of substrates 7,8-dihydrofolate and NADPH for the 2 isozymes | Escherichia coli |