Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chaotrophes | e.g. guanidine-HCl; formamide; urea | Crithidia fasciculata | |
folate | - |
Crithidia fasciculata | |
L-erythro-biopterin | - |
Crithidia fasciculata | |
methotrexate | - |
Crithidia fasciculata | |
N-ethylmaleimide | - |
Crithidia fasciculata | |
NADP+ | - |
Crithidia fasciculata | |
p-chloromercuribenzoate | - |
Crithidia fasciculata | |
pyrimethamine | - |
Crithidia fasciculata | |
trimethoprim | - |
Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0009 | - |
dihydropteroate | - |
Crithidia fasciculata | |
0.0021 | - |
NADPH | with dihydropteroate | Crithidia fasciculata | |
0.0034 | - |
6-Hydroxymethylpterin | - |
Crithidia fasciculata | |
0.0035 | - |
L-threo-neopterin | - |
Crithidia fasciculata | |
0.0048 | - |
7,8-dihydrofolate | - |
Crithidia fasciculata | |
0.0059 | - |
NADPH | with 7,8-dihydrofolate or 6-hydroxymetylpterin | Crithidia fasciculata | |
0.011 | - |
NADPH | with L-threo-neopterin | Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
gel filtration | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
bifunctional enzyme: pteridine reductase-dihydrofolate reductase | - |
Purification (Comment) | Organism |
---|---|
bifunctional enzyme: pteridine reductase-dihydrofolate reductase | Crithidia fasciculata |
Storage Stability | Organism |
---|---|
ammonium sulfate, 55% saturation, 0°C, for at least 1 year | Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxymethylpterin + NADPH | - |
Crithidia fasciculata | 6-hydroxymethyl-7,8-dihydropterin + NADP+ | - |
? | |
6-methylpterin + NADPH | - |
Crithidia fasciculata | 6-methyl-7,8-dihydropterin + NADP+ | - |
? | |
7,8-dihydrofolate + NADPH | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
D-erythro-7,8-neopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
D-threo-neopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
dihydropteroate + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
folate + NADPH | no activity | Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
L-erythro-neopterin + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
L-threo-7,8-dihydroneopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? | |
L-threo-neopterin + NADPH | - |
Crithidia fasciculata | ? | - |
? | |
additional information | no activity with folate, sepiapterin, isoxanthopterin, leucopterin | Crithidia fasciculata | ? | - |
? | |
pterin + NADPH | low activity | Crithidia fasciculata | 7,8-dihydropterin + NADP+ | - |
? | |
xanthopterin + NADPH | low activity | Crithidia fasciculata | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Crithidia fasciculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | not | Crithidia fasciculata | |
NADPH | - |
Crithidia fasciculata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
L-erythro-biopterin | with 7,8-dihydrofolate | Crithidia fasciculata | |
0.00072 | - |
pyrimethamine | with 7,8-dihydrofolate | Crithidia fasciculata | |
0.0011 | - |
methotrexate | with 7,8-dihydrofolate | Crithidia fasciculata | |
0.0064 | - |
trimethoprim | with 7,8-dihydrofolate | Crithidia fasciculata | |
0.021 | - |
NADP+ | with 7,8-dihydrofolate | Crithidia fasciculata | |
0.036 | - |
folate | with 7,8-dihydrofolate | Crithidia fasciculata |