KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km of membrane-bound and Triton X-100-solubilized enzyme | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | negligible amounts in the purified enzym | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
gel filtration, lowest molecular weight determined, variations due to different states of aggregation | Sus scrofa |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | when enzyme liberated by methyl ethyl ketone extraction Km about 3times lower than that for enzyme bound to the mitochondria or solubilzed by Triton X-100 | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | enzyme is bound to the mitochondria by acidic phospholipids, soluble enzyme can form complexes with such phospholipids | Sus scrofa |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzylamine + H2O + O2 | - |
Sus scrofa | benzaldehyde + NH3 + H2O2 | - |
? | |
kynuramine + H2O + O2 | - |
Sus scrofa | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with spermidine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with histamine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | no activity with spermine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | phenylethylhydrazine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | isoamylamine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | 3-hydroxytyramine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | amylamine | Sus scrofa | RCHO + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | - |
Sus scrofa | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tryptamine + H2O + O2 | - |
Sus scrofa | 1H-indol-3-yl-acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Sus scrofa | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
tyramine as substrate | Sus scrofa |
9.2 | - |
benzylamine as substrate | Sus scrofa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 11 | about 50% of activity maximum at pH 7 and 11 | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Sus scrofa | |
flavin | 1 mol FAD per 115000 g of protein | Sus scrofa |