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Literature summary for 1.4.3.3 extracted from
- Is rat an appropriate animal model to study the involvement of D-serine catabolism in schizophrenia? Insights from characterization of D-amino acid oxidase (2011), FEBS J., 278, 4362-4373.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)Star cells | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-chlorobenzo(d)isoxazol-3-ol | - |
Rattus norvegicus |  |
| anthranilate | - |
Rattus norvegicus | |
| benzoate | - |
Rattus norvegicus | |
| chlorpromazine | FAD-competitive inhibitor | Rattus norvegicus | |
| crotonate | - |
Rattus norvegicus | |
| sulfite | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
O2 | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 15 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 35 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 86 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 140 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 310 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
1 * 40000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-amino acid + H2O + O2 | Rattus norvegicus | - |
2-oxocarboxylate + NH3 + H2O2 | - |
? | |
| additional information | Rattus norvegicus | the enzyme interacts with the mammalian protein modulator pLG72 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-chelate affinity column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | - |
Rattus norvegicus | pyruvate + NH3 + H2O2 | - |
? | |
| D-amino acid + H2O + O2 | - |
Rattus norvegicus | 2-oxocarboxylate + NH3 + H2O2 | - |
? | |
| D-phenylalanine + H2O + O2 | - |
Rattus norvegicus | phenylpyruvate + NH3 + H2O2 | - |
? | |
| D-proline + H2O + O2 | - |
Rattus norvegicus | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
| D-serine + H2O + O2 | - |
Rattus norvegicus | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
| D-tryptophan + H2O + O2 | - |
Rattus norvegicus | indol-3-pyruvate + NH3 + H2O2 | - |
? | |
| additional information | the enzyme interacts with the mammalian protein modulator pLG72 | Rattus norvegicus | ? | - |
? | |
| additional information | D-aspartate and glycine are no substrates | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-amino acid oxidase | - |
Rattus norvegicus |
| DAAO | - |
Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.7 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 6.4 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 8.5 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 27 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 40 | - |
O2 | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 47 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
benzoate | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 1.6 | - |
anthranilate | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
D-serine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 0.2 | - |
D-alanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 0.24 | - |
D-phenylalanine | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 0.25 | - |
D-tryptophan | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
| 0.55 | - |
D-proline | in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C | Rattus norvegicus | |
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