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Literature summary for 1.4.3.3 extracted from

  • Caldinelli, L.; Molla, G.; Sacchi, S.; Pilone, M.S.; Pollegioni, L.
    Relevance of weak flavin binding in human D-amino acid oxidase (2009), Protein Sci., 18, 801-810.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme expression in Escherichia coli Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, stable homodimer even in the apoprotein form Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens DAAO is a catabolic flavoenzyme that catalyzes the oxidative deamination of D-amino acids to the corresponding a-keto acids, hydrogen peroxide, and ammonia. DAAO is strictly specific for D-isomers of amino acids ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
flavoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
holo- and apoprotein samples of hDAAO are incubated for 60 min at 15°C in a buffer containing different concentrations of urea, 0-6 M, and then refolded by 10fold dilution in 50 mM sodium diphosphate, pH 8.0, 5% glycerol and 15°C, in the presence of a 10fold molar excess of FAD. Chemical denaturation of hDAAO holoenzyme is partially reversible, 50% of the initial activity is recovered starting with the refolding from 4 M urea-denatured holoprotein, while the refolding of apoprotein is largely irreversible even at 2 M urea , 15-20% of recovery of enzymatic activity versus 90-100% for the holoenzyme and even in the presence of the cofactor Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DAAO is a catabolic flavoenzyme that catalyzes the oxidative deamination of D-amino acids to the corresponding a-keto acids, hydrogen peroxide, and ammonia. DAAO is strictly specific for D-isomers of amino acids Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 40000, stable homodimer even in the apoprotein form Homo sapiens
More hDAAO exists as an equilibrium of holo- and apoprotein forms in solution Homo sapiens

Synonyms

Synonyms Comment Organism
DAAO
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme, weak interaction with the flavin cofactor in the free form,the cofactor binding is significantly tighter in the presence of an active site ligand. hDAAO exists as an equilibrium of holo- and apoprotein forms in solution Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens the apoprotein gives a single band whose pI of 6.3 is very close to the theoretical value of 6.36, the holoenzyme yields three bands at pI 6.3, 5.9, and 5.4 6.3 5.4

General Information

General Information Comment Organism
physiological function DAAO is involved in the degradation of the gliotransmitter D-serine, an important modulator of NMDA-receptor-mediated neurotransmission. An increase in DAAO activity, yielding a decrease in D-serine concentration, is among the molecular mechanisms leading to the onset of schizophrenia susceptibility, overview Homo sapiens