Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
D-alanine | free enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C | Rhodotorula toruloides | |
1.6 | - |
D-alanine | immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C | Rhodotorula toruloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, SDS-PAGE | Rhodotorula toruloides |
80000 | - |
SDS-PAGE | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Oxidation Stability | Organism |
---|---|
the immobilized enzyme (onto Ni2+-chelated NTA magnetic beads) does not show any loss of activity in the presence of 10 mM H2O2 after 2 h incubation which is observed in case of free enzyme, the residual activity of the immoblized enzyme after 9 h is 72% compared with 22% of the free enzyme | Rhodotorula toruloides |
Purification (Comment) | Organism |
---|---|
His-Bind column chromatography | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
22°C, free enzyme, 10 days, 20% residual activity | Rhodotorula toruloides |
22°C, immobilized enzyme, 10 days, 74% residual activity | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cephalosporin C + H2O + O2 | - |
Rhodotorula toruloides | 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2 | - |
? | |
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 40000, SDS-PAGE | Rhodotorula toruloides |
Synonyms | Comment | Organism |
---|---|---|
D-amino acid oxidase | - |
Rhodotorula toruloides |
DAO | - |
Rhodotorula toruloides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
free and immobilized enzymes | Rhodotorula toruloides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the native enzyme is inacivated after 1 h at 50°C whereas the immobilized enzyme (onto Ni2+-chelated NTA magnetic beads) retains 56% of the initial activity | Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.3 | - |
D-alanine | immobilized enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C | Rhodotorula toruloides | |
52.4 | - |
D-alanine | free enzyme, in 100 mM potassium phosphate buffer at pH 8.0 and 25°C | Rhodotorula toruloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
free enzyme | Rhodotorula toruloides |
9 | - |
immobilized enzyme | Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | at pH 8 immobilized enzyme (onto Ni2+-chelated NTA magnetic beads) activity decreases to 44% while the free enzyme showed optimal activity, the activities of both enzyme forms decay similarily at pH values above 9, the free enzyme activity is less affected for pH values below 9 than the immobilized enzyme | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodotorula toruloides |