Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.3 extracted from

  • Boselli, A.; Piubelli, L.; Molla, G.; Pilone, M.S.; Pollegioni, L.; Sacchi, S.
    Investigating the role of active site residues of Rhodotorula gracilis D-amino acid oxidase on its substrate specificity (2007), Biochimie, 89, 360-368.
    View publication on PubMed
Search for more literature for 1.4.3.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodotorula toruloides

Protein Variants

Protein Variants Comment Organism
M213E mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
M213R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Q339E mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Q339N mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
R285A/D/K/Q inactive Rhodotorula toruloides
S335G mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
S335H mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
S335R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Y223F mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Y223S mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Y238F mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Y238R mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides
Y238S mutant shows lower activity on neutral and basic amino acids compared to the wild type enzyme Rhodotorula toruloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
 D-alanine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.4
-
 D-alanine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.4
-
 D-alanine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.4
-
 D-alanine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.5
-
 D-alanine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.6
-
 D-alanine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.7
-
 D-alanine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.8
-
 D-alanine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1
-
 cephalosporin C mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1
-
 cephalosporin C mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.1
-
 D-alanine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.1
-
 D-alanine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.3
-
 D-arginine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.3
-
 D-alanine mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.7
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.9
-
 cephalosporin C mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.9
-
 cephalosporin C mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2
-
 D-Aspartate mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2 3 D-arginine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.5
-
 D-arginine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.5
-
 cephalosporin C mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.8
-
 cephalosporin C mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.8
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
4.3
-
 cephalosporin C mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
5
-
 cephalosporin C wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
5.2
-
 D-Aspartate mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
5.5
-
 D-arginine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
5.5
-
 D-arginine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
6.7
-
 D-arginine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
8.7
-
 cephalosporin C mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
9.2
-
 D-arginine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
9.7
-
 D-arginine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
13
-
 cephalosporin C mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
13
-
 D-arginine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
15
-
 D-Aspartate mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
15
-
 D-Aspartate mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
17
-
 D-Aspartate mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
18
-
 D-alanine mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
18
-
 D-Aspartate wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
18
-
 D-arginine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
28
-
 cephalosporin C mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
29
-
 D-arginine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
30
-
 D-Aspartate mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
33
-
 D-Aspartate mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
40
-
 D-alanine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
53
-
 D-Aspartate mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
61
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
72
-
 D-Aspartate mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
98
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
150
-
 D-Aspartate mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 SDS-PAGE Rhodotorula toruloides

Organism

Organism UniProt Comment Textmining
Rhodotorula toruloides P80324
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose column chromatography Rhodotorula toruloides

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
104
-
 purified wild type enzyme, at 25°C Rhodotorula toruloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
 Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
 ?
D-alanine + H2O + O2
-
 Rhodotorula toruloides pyruvate + NH3 + H2O2
-
 ?
D-arginine + H2O + O2
-
 Rhodotorula toruloides 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
 ?
D-aspartate + H2O + O2
-
 Rhodotorula toruloides oxaloacetate + NH3 + H2O2
-
 ?
additional information the wild-type DAAO is mainly active on neutral D-amino acids, while basic D-amino acids are poor substrates and the acidic ones are virtually not oxidized Rhodotorula toruloides ?
-
 ?

Synonyms

Synonyms Comment Organism
D-amino acid oxidase
-
 Rhodotorula toruloides
DAAO
-
 Rhodotorula toruloides

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 D-Aspartate mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.2
-
 D-arginine kcat less than 0.2/s, mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.26
-
 D-Aspartate mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.37
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.43
-
 D-arginine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.5
-
 D-Aspartate mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.5
-
 D-Aspartate wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.56
-
 D-Aspartate mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.63
-
 D-alanine mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.67
-
 D-Aspartate mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.79
-
 D-alanine mutant enzyme Q339N, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
0.9
-
 D-Aspartate mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1
-
 D-Aspartate mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
1.3
-
 cephalosporin C mutant enzyme Y223S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.1
-
 D-arginine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.5
-
 D-Aspartate mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
2.6
-
 D-arginine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.2
-
 D-Aspartate mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.2
-
 D-arginine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.6
-
 cephalosporin C mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.6
-
 D-arginine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.7
-
 D-alanine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
3.9
-
 D-Aspartate mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
4.4
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
6
-
 D-Aspartate mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
6.5
-
 D-arginine mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
6.9
-
 D-alanine mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
7
-
 cephalosporin C mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
7.3
-
 cephalosporin C mutant enzyme M213E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
7.3
-
 D-arginine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
7.8
-
 cephalosporin C mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
8.6
-
 D-arginine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
9.2
-
 D-alanine mutant enzyme S335R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
10
-
 D-alanine mutant enzyme M213R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
11
-
 D-arginine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
14
-
 D-arginine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
15
-
 D-alanine mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
15
-
 cephalosporin C mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
17
-
 cephalosporin C mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
20
-
 D-arginine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
24
-
 cephalosporin C mutant enzyme Q339E, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
24
-
 cephalosporin C mutant enzyme Y238R, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
25
-
 D-alanine mutant enzyme Y228F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
25
-
 D-alanine mutant enzyme Y238S, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
26
-
 cephalosporin C mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
48
-
 D-alanine mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
50
-
 cephalosporin C mutant enzyme S335G, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
67
-
 D-alanine mutant enzyme Y223F, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
72
-
 cephalosporin C wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
75
-
 D-alanine mutant enzyme S335H, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides
81
-
 D-alanine wild type enzyme, in 50 mM sodium diphosphate, pH 8.5, at air (21%) oxygen saturation, at 25°C, apparent value Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Rhodotorula toruloides