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Literature summary for 1.4.3.3 extracted from
- Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring (2006), Protein Sci., 15, 2708-2717.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method, crystal structure of the enzyme in complex with the competitive inhibitor benzoate at a resolution of 2.5 A | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| homodimer is stable even in the apoprotein form | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| benzoate | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
D-Ala | pH 8.3, 25°C | Homo sapiens | |
| 1.7 | - |
D-Pro | pH 8.3, 25°C | Homo sapiens | |
| 3.6 | - |
D-Ser | pH 8.3, 25°C | Homo sapiens | |
| 140 | - |
Gly | pH 8.3, 25°C | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Ala + H2O + O2 | - |
Homo sapiens | pyruvate + NH3 + H2O2 | - |
? | |
| D-Pro + H2O + O2 | - |
Homo sapiens | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
| D-Ser + H2O + O2 | - |
Homo sapiens | 2-oxo-3-hydroxypropionate + NH3 + H2O2 | - |
? | |
| Gly + H2O + O2 | - |
Homo sapiens | acetate + NH3 + H2O2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DAO | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
Gly | pH 8.3, 25°C | Homo sapiens | |
| 2.83 | - |
D-Ser | pH 8.3, 25°C | Homo sapiens | |
| 5.5 | - |
D-Ala | pH 8.3, 25°C | Homo sapiens | |
| 15 | - |
D-Pro | pH 8.3, 25°C | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | human enzyme weakly binds FAD and shows a significantly slower rate of flavin reduction compared to porcine enzyme | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
benzoate | - |
Homo sapiens | |
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