Protein Variants | Comment | Organism |
---|---|---|
S335G | mutant enzyme has a lower turnover number with D-Ala than wild-type enzyme. The spectral and ligand binding properties of the mutant are similar to those of the wild-type enzyme | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
O2 | pH 8.5, 25°C, mutant enzyme S335G, reaction with D-Ala | Rhodotorula toruloides | |
0.8 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme, at | Rhodotorula toruloides | |
1.1 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme S335G | Rhodotorula toruloides | |
2.3 | - |
O2 | pH 8.5, 25°C, wild-type enzyme, reaction with D-Ala | Rhodotorula toruloides | |
18 | - |
D-Asp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
30 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme S335G | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-Asp + H2O + O2 | - |
Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RgDAAO | - |
Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
82 | - |
O2 | pH 8.5, 25°C, mutant enzyme S335G | Rhodotorula toruloides | |
82 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme S335G | Rhodotorula toruloides | |
350 | - |
O2 | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
350 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |