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Literature summary for 1.4.3.3 extracted from

  • Pilone, M.S.
    D-Amino acid oxidase: new findings (2000), Cell. Mol. Life Sci., 57, 1732-1747.
    View publication on PubMed

Application

Application Comment Organism
medicine human gene therapy Rhodotorula toruloides
medicine human gene therapy Trigonopsis variabilis
synthesis oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid Rhodotorula toruloides
synthesis oxidation of cephalosporin C in the two-step formation of 7-aminocephalosporanic acid Trigonopsis variabilis

Cloned(Commentary)

Cloned (Comment) Organism
-
Rhodotorula toruloides
expression in Kluyveromyces lactis Trigonopsis variabilis
expression in Saccharomyces cerevisiae Trigonopsis variabilis

Protein Variants

Protein Variants Comment Organism
Y223F slower substrate binding than the wild-type Trigonopsis variabilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
alpha2, 2 * 40000, SDS-PAGE Trigonopsis variabilis
79000
-
-
Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cephalosporin C + H2O + O2 Rhodotorula toruloides
-
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
?
cephalosporin C + H2O + O2 Trigonopsis variabilis
-
7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rhodotorula toruloides
-
-
-
Sus scrofa
-
-
-
Trigonopsis variabilis
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Homo sapiens
-
kidney
-
Sus scrofa
-
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cephalosporin C + H2O + O2
-
Rhodotorula toruloides 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
?
cephalosporin C + H2O + O2
-
Trigonopsis variabilis 7-(5-oxoadipoamido)cephalosporanic acid + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer
-
Rhodotorula toruloides
dimer alpha2, 2 * 40000, SDS-PAGE Trigonopsis variabilis
monomer
-
Sus scrofa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
D-alanine
-
Rhodotorula toruloides
0.8
-
D-alanine
-
Rhodotorula toruloides
4.2
-
D-alanine mutant Y223S Rhodotorula toruloides
10
-
D-alanine
-
Homo sapiens
10
-
D-alanine
-
Sus scrofa
210
-
D-alanine mutant Y223F Rhodotorula toruloides
345
-
D-alanine
-
Rhodotorula toruloides
350
-
D-alanine
-
Rhodotorula toruloides
737
-
cephalosporin C
-
Rhodotorula toruloides

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Homo sapiens
FAD flavoprotein Sus scrofa
FAD flavoprotein Rhodotorula toruloides
FAD flavoprotein Trigonopsis variabilis