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Literature summary for 1.4.1.4 extracted from

  • Lee, Y.J.; Kim, K.J.; Kang, H.Y.; Kim, H.R.; Maeng, P.J.
    Involvement of GDH3-encoded NADP+-dependent glutamate dehydrogenase in yeast cell resistance to stress-induced apoptosis in stationary phase cells (2012), J. Biol. Chem., 287, 44221-44233.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
genes GDH1 and GDH3, recombinant expression of FLAG-tagged wild-type and mutant GDH1 and GDH3 in yeast cells Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K419A site-directed mutagenesis of GDH1 Saccharomyces cerevisiae
K420A site-directed mutagenesis of GDH1 Saccharomyces cerevisiae
K423A site-directed mutagenesis of GDH1 Saccharomyces cerevisiae
K426A site-directed mutagenesis of GDH1 Saccharomyces cerevisiae
additional information promoter swapping and site-directed mutagenesis of GDH1 and GDH3, construction of gene disruption null mutants of both genes, phenotypes and mutant activities, overview Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NADP+ Saccharomyces cerevisiae
-
2-oxoglutarate + NH3 + NADPH + H+
-
r
L-glutamate + H2O + NADP+ Saccharomyces cerevisiae BY4741
-
2-oxoglutarate + NH3 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
two NADP+-dependent glutamate dehydrogenases encoded by GDH1 and GDH3
-
Saccharomyces cerevisiae BY4741
-
two NADP+-dependent glutamate dehydrogenases encoded by GDH1 and GDH3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
Saccharomyces cerevisiae 2-oxoglutarate + NH3 + NADPH + H+
-
r
L-glutamate + H2O + NADP+
-
Saccharomyces cerevisiae BY4741 2-oxoglutarate + NH3 + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
NADP+-dependent glutamate dehydrogenase
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Saccharomyces cerevisiae
NADP-GDH
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Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Saccharomyces cerevisiae
NADPH
-
Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction Gdh3-null cells show accelerated chronological aging and hypersusceptibility to thermal and oxidative stress during stationary phase. Upon exposure to oxidative stress, Gdh3-null strains display a rapid loss in viability associated with typical apoptotic hallmarks, i.e. reactive oxygen species accumulation, nuclear fragmentation, DNA breakage, and phosphatidylserine translocation. In addition, Gdh3-null cells, but not Gdh1-null cells, have a higher tendency toward GSH depletion and subsequent reactive oxygen species accumulation than did wild-type cells. GSH depletion is rescued by exogenous GSH or glutamate. The hypersusceptibility of stationary phase Gdh3-null cells to stress-induced apoptosis is suppressed by deletion of GDH2. Gdh1, but not Gdh3, is subjected to stationary phase-specific degradation in which the Lys-426 residue in the Box420Gdh1 region plays an essential role Saccharomyces cerevisiae
physiological function involvement of GDH3-encoded NADP+-dependent glutamate dehydrogenase in yeast cell resistance to stress-induced apoptosis in stationary phase cells, overview. GDH1, but not GDH3, is responsible for the resistance against stress-induced apoptosis in logarithmic phase cells, Necessity of GDH3 for the resistance to stress-induced apoptosis and chronological aging is due to the stationary phase-specific expression of GDH3 and concurrent degradation of Gdh1 in which the Lys-426 residue plays an essential role Saccharomyces cerevisiae