General Stability | Organism |
---|---|
2-mercaptoethanol and dithiothreitol stabilizes | Corallomycetella repens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
NADP+ | oxidative deamination | Corallomycetella repens | |
0.049 | - |
NADPH | reductive amination | Corallomycetella repens | |
0.64 | - |
2-oxoglutarate | reductive amination | Corallomycetella repens | |
2.6 | - |
NH4+ | reductive amination, biphasic kinetics | Corallomycetella repens | |
5.5 | - |
L-glutamate | oxidative deamination | Corallomycetella repens | |
21.2 | - |
NH4+ | reductive amination | Corallomycetella repens | |
21.2 | - |
NH4+ | biphasic kinetics | Corallomycetella repens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
alpha6, 6 * 48000, SDS-PAGE | Corallomycetella repens |
280000 | - |
non-denaturating PAGE | Corallomycetella repens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Corallomycetella repens | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corallomycetella repens | - |
- |
- |
Storage Stability | Organism |
---|---|
4°C, ten days and 25°C, 15 h, both 100% activity | Corallomycetella repens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Corallomycetella repens | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Corallomycetella repens | 2-oxoglutarate + NADPH + NH3 | - |
r | |
L-glutamate + NADP+ + H2O | - |
Corallomycetella repens | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 48000, SDS-PAGE | Corallomycetella repens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
stable for 15 h | Corallomycetella repens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1100 | - |
NADPH | - |
Corallomycetella repens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
reductive amination | Corallomycetella repens |
8.4 | - |
oxidative deamination | Corallomycetella repens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Corallomycetella repens | |
NADPH | - |
Corallomycetella repens |