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Literature summary for 1.4.1.4 extracted from
- NADP+-dependent glutamate dehydrogenase from Acropora formosa (1987), Mar. Biol., 95, 559-563.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| inactivated by freezing, less than 5% activity after one freeze/thaw cycle | Acropora formosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
NADP+ | oxidative deamination | Acropora formosa |  |
| 0.11 | - |
NADPH | reductive amination | Acropora formosa | |
| 0.93 | - |
2-oxoglutarate | reductive amination | Acropora formosa | |
| 9.2 | - |
NH4+ | reductive amination | Acropora formosa | |
| 18 | - |
L-glutamate | oxidative deamination, biphasic kinetics | Acropora formosa | |
| 81 | - |
L-glutamate | oxidative deamination, biphasic kinetics | Acropora formosa | |
| 416 | - |
NH4+ | reductive amination | Acropora formosa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56000 | - |
alpha6, 6 * 56000, SDS-PAGE | Acropora formosa |
| 360000 | - |
gel filtration | Acropora formosa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Acropora formosa | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acropora formosa | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C after 7 days, 55% activity | Acropora formosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Acropora formosa | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Acropora formosa | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Acropora formosa | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for ammonia | r | |
| L-glutamate + NADP+ + H2O | - |
Acropora formosa | 2-oxoglutarate + NADPH + NH3 | biphasic kinetic behavior for L-glutamate | r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 56000, SDS-PAGE | Acropora formosa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
after 25 min 10% activity | Acropora formosa |
| 75 | - |
after 2 min 50% activity | Acropora formosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 7.6 | oxidative deamination | Acropora formosa |
| 9.6 | 10 | reductive amination | Acropora formosa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Acropora formosa | |
| NADPH | - |
Acropora formosa | |
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Release 2023.1 (January 2023)
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