Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | at 20 mM, 25% and 108% activation of reductive amination and oxidative deamination respectively | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | reductive amination | Synechocystis sp. | |
1.5 | - |
2-oxoglutarate | reductive amiantion | Synechocystis sp. | |
3.7 | - |
NH4+ | reductive amination | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
alpha4, 4 * 46000, SDS-PAGE | Synechocystis sp. |
208000 | - |
gel filtration | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Synechocystis sp. | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
strain PCC 6803 | - |
Purification (Comment) | Organism |
---|---|
- |
Synechocystis sp. |
Storage Stability | Organism |
---|---|
0-4°C, Tris-HCl buffer, pH 9.0, 2 months, 100% activity | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Synechocystis sp. | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Synechocystis sp. | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | alpha4, 4 * 46000, SDS-PAGE | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | reductive amination | Synechocystis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Synechocystis sp. | |
NADPH | - |
Synechocystis sp. |