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Literature summary for 1.4.1.4 extracted from

  • Bascomb, N.F.; Schmidt, R.R.
    Purification and partial kinetic and physical characterization of two chloroplast-localized NADP-specific glutamate dehydrogenase isoenzymes and their preferential accumulation in Chlorella sorokiniana cells cultured at low or high ammonium levels (1987), Plant Physiol., 83, 75-84.
    View publication on PubMedView publication on EuropePMC
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General Stability

General Stability Organism
purification under anoxically conditions, 2 mM dithiothreitol stabilizes Chlorella sorokiniana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 NADP+ oxidative deamination Chlorella sorokiniana
0.04
-
 NADP+ oxidative deamination Chlorella sorokiniana
3.3
-
 NH4+ reductive amination alpha-isoenzyme, depending on NADPH-concentration Chlorella sorokiniana
32.3
-
 L-glutamate oxidative deamination Chlorella sorokiniana
38.2
-
 L-glutamate oxidative deamination Chlorella sorokiniana
75
-
 NH4+ reductive amination beta-isoenzyme Chlorella sorokiniana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Chlorella sorokiniana 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 x * 53000, SDS-PAGE, beta-holoenzyme Chlorella sorokiniana
55500
-
 x * 55500, SDS-PAGE, alpha-holoenzyme Chlorella sorokiniana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Chlorella sorokiniana
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Chlorella sorokiniana
-
 two isoenzymes alpha-subunits at 2 mM ammonia and beta-subunits at 29 mM ammonia
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chlorella sorokiniana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
 Chlorella sorokiniana L-glutamate + NADP+
-
 ?
L-glutamate + NADP+ + H2O
-
 Chlorella sorokiniana 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
? x * 55500, SDS-PAGE, alpha-holoenzyme Chlorella sorokiniana
? x * 53000, SDS-PAGE, beta-holoenzyme Chlorella sorokiniana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Chlorella sorokiniana
NADPH
-
 Chlorella sorokiniana