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Literature summary for 1.4.1.4 extracted from
- Ion pairs involved in maintaining a thermostable structure of glutamate dehydrogenase from a hyperthermophilic archaeon (1998), Biochem. Biophys. Res. Commun., 248, 920-926.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermococcus kodakarensis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E158Q | 3.3% as active as wild-type | Thermococcus kodakarensis |
| T138E | 1.6% as active as wild-type | Thermococcus kodakarensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47300 | - |
alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
| 284000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Thermococcus kodakarensis | - |
2-oxoglutarate + NADPH + NH3 | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus kodakarensis | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 40 | - |
T138E-mutant | Thermococcus kodakarensis |
| 80 | - |
E158Q-mutant | Thermococcus kodakarensis |
| 2500 | - |
wild-type | Thermococcus kodakarensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Thermococcus kodakarensis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | alpha6, 6 * 47300, SDS-PAGE | Thermococcus kodakarensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
E158Q-mutant | Thermococcus kodakarensis |
| 85 | - |
T138E-mutant | Thermococcus kodakarensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
wild-type and T138E 50% activity after 2 h and 3 h respectively | Thermococcus kodakarensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Thermococcus kodakarensis | |
| NADPH | - |
Thermococcus kodakarensis | |
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Release 2023.1 (January 2023)
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