Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | activates | Aeropyrum pernix K1 | |
ethanol | activates up to 40% v/v | Aeropyrum pernix K1 | |
methanol | activates | Aeropyrum pernix K1 |
General Stability | Organism |
---|---|
1 mg/ml bovine serum albumin stabilizes | Aeropyrum pernix K1 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | complete loss of activity | Aeropyrum pernix K1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.049 | - |
NADPH | reductive amination | Aeropyrum pernix K1 | |
0.053 | - |
NADP+ | oxidative deamination | Aeropyrum pernix K1 | |
0.32 | - |
2-oxoglutarate | reductive amination | Aeropyrum pernix K1 | |
4.56 | - |
NH3 | reductive amination | Aeropyrum pernix K1 | |
5.18 | - |
L-glutamate | oxidative deamination | Aeropyrum pernix K1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
alpha6, 6 * 47000, SDS-PAGE | Aeropyrum pernix K1 |
285000 | - |
gel filtration | Aeropyrum pernix K1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Aeropyrum pernix K1 | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix K1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Aeropyrum pernix K1 | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + NADP+ + H2O | - |
Aeropyrum pernix K1 | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 47000, SDS-PAGE | Aeropyrum pernix K1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
reductive amination | Aeropyrum pernix K1 |
95 | - |
oxidative deamination | Aeropyrum pernix K1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
74% activity after 5 h | Aeropyrum pernix K1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reductive amination and oxidative deamination | Aeropyrum pernix K1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | above pH 9 or below 4.0 no activity | Aeropyrum pernix K1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Aeropyrum pernix K1 | |
NADPH | - |
Aeropyrum pernix K1 |