Activating Compound | Comment | Organism | Structure |
---|---|---|---|
K2HPO4 | at 0.15 M three to fourfold stimulation of activity | Archaeoglobus fulgidus | |
KCl | at 0.2 M three to fourfold stimulation of activity | Archaeoglobus fulgidus | |
NaCl | at 0.2 M three to fourfold stimulation of activity | Archaeoglobus fulgidus |
General Stability | Organism |
---|---|
potassium phosphate enhances the thermostability | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADPH | reductive amination at 60°C | Archaeoglobus fulgidus | |
0.06 | - |
NADP+ | oxidative deamination at 60°C | Archaeoglobus fulgidus | |
3.9 | - |
L-glutamate | oxidative deamination at 60°C | Archaeoglobus fulgidus | |
4 | - |
NH3 | reductive amination at 60°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
alpha6, 6 * 47000, SDS-PAGE | Archaeoglobus fulgidus |
263000 | - |
gel filtration | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Archaeoglobus fulgidus | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
additional information | Archaeoglobus fulgidus | glutamate dehydrogenase functions physiologically for the synthesis of L-glutamate from 2-oxoglutarate and ammonia | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
strain DSM 8774 | - |
Purification (Comment) | Organism |
---|---|
under semianoxically conditions | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Archaeoglobus fulgidus | L-glutamate + H2O + NAD+ | - |
r | |
L-glutamate + NADP+ + H2O | - |
Archaeoglobus fulgidus | 2-oxoglutarate + NADPH + NH3 | - |
r | |
additional information | glutamate dehydrogenase functions physiologically for the synthesis of L-glutamate from 2-oxoglutarate and ammonia | Archaeoglobus fulgidus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 47000, SDS-PAGE | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
reductive amination | Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
fully active after 5 h | Archaeoglobus fulgidus |
100 | - |
50% activity after 140 min | Archaeoglobus fulgidus |
105 | - |
50% activity after 20 min | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
oxidative deamination at 60°C | Archaeoglobus fulgidus |
8.4 | - |
reductive amination at 60°C | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Archaeoglobus fulgidus | |
NADPH | - |
Archaeoglobus fulgidus |