Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | ADP induces an allosteric conformational change in GDH1, leading to a 2fold enhanced oxidative deamination | Rattus norvegicus | |
leucine | enhances the oxidative deamination reaction of GDH1 | Rattus norvegicus | |
PMP-BCATm | pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) accelerates the oxidative deamination reaction of GDH1in the presence of branched-chain amino acids (Leu, Ile, Val). Reductive amination reaction is not affected | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GDP | strong allosteric inhibitor of GDH1 leading to a 90% reduction of activity. Addition of increasing concentrations of pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm) leads to an increasing protection from GDP inhibition | Rattus norvegicus | |
GTP | - |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P10860 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
pancreas | - |
Rattus norvegicus | - |
skeletal muscle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NAD+ + H2O | - |
Rattus norvegicus | 2-oxoglutarate + NADH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | method of determination not further specified | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
GDH1 | - |
Rattus norvegicus |
glutamate dehydrogenase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reductive amination at | Rattus norvegicus |
8 | - |
oxidative deamination at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | GDH1 uses NAD+ or NADP+ as coenzyme | Rattus norvegicus | |
NADP+ | GDH1 uses NAD+ or NADP+ as coenzyme | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
GTP | GTP alone | Rattus norvegicus | |
0.00156 | - |
GTP | molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 1 | Rattus norvegicus | |
0.00312 | - |
GTP | molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 2 | Rattus norvegicus | |
0.00795 | - |
GTP | molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 6 | Rattus norvegicus | |
0.00806 | - |
GTP | molar ratio pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm)/GDH1: 4 | Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
metabolism | GDH1 is the protein partner for pyridoxamine 5'-phosphate-form of the mitochondrial branched chain aminotransferase (PMP-BCATm). Facilitating the recycling of BCATm to form metabolon, GDH1 acts as a catalytic machine | Rattus norvegicus |