Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | 0.1-1.0 mM, activates wild-type enzyme and mutant enzyme E279G | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of Glu279 mutants in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E279G | 9.8fold increase in Km-value for NAD+ | Homo sapiens |
E279L | 14.1fold increase in Km-value for NAD+ | Homo sapiens |
E279M | 14.1fold increase in Km-value for NAD+ | Homo sapiens |
E279R | 10.7fold increase in Km-value for NAD+ | Homo sapiens |
E279Y | 11.8fold increase in Km-value for NAD+ | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | inhibits wild-type enzyme and mutant enzyme E279G | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
NAD+ | pH 9.5, 25°C, wild-type enzyme | Homo sapiens | |
3.44 | - |
L-glutamate | pH 9.5, 25°C, wild-type enzyme | Homo sapiens | |
3.76 | - |
L-glutamate | pH 9.5, 25°C, mutant enzyme E279M | Homo sapiens | |
3.94 | - |
L-glutamate | pH 9.5, 25°C, mutant enzyme E279Y | Homo sapiens | |
3.98 | - |
L-glutamate | pH 9.5, 25°C, mutant enzyme E279G | Homo sapiens | |
4.05 | - |
L-glutamate | pH 9.5, 25°C, mutant enzyme E279L | Homo sapiens | |
4.12 | - |
L-glutamate | pH 9.5, 25°C, mutant enzyme E279R | Homo sapiens | |
6.94 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279G | Homo sapiens | |
7.6 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279R | Homo sapiens | |
8.35 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279Y | Homo sapiens | |
9.98 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279M | Homo sapiens | |
10.01 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279L | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and Glu279 mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NAD+ + H2O | - |
Homo sapiens | 2-oxoglutarate + NADH + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
51 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279M | Homo sapiens | |
57 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279L | Homo sapiens | |
59 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279G | Homo sapiens | |
61 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279R | Homo sapiens | |
65 | - |
NAD+ | pH 9.5, 25°C, wild-type enzyme | Homo sapiens | |
68 | - |
NAD+ | pH 9.5, 25°C, mutant enzyme E279Y | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |