Protein Variants | Comment | Organism |
---|---|---|
G446D | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
H454Y | mutation results in depolymerization of hexameric enzyme into active trimers. Mutation has no effect on expression or stability of the protein. The Km-value for NADH is 1.5fold greater than the wild-type value and the KM-value for 2-oxoglutarate is 2.5fold greater than the wild-type value. Vmax values are similar for wild-type and mutant enzyme | Homo sapiens |
K333L | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
K337L | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
K344L | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
K346L | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
S445L | kinetic parameters are almost identical to that of the wild-type enzyme. Subunit composition and polymerisation process are not affected by matagenesis | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.076 | - |
NADH | 25°C, mutant enzyme K346L | Homo sapiens | |
0.079 | - |
NADH | 25°C, mutant enzyme K333L | Homo sapiens | |
0.081 | - |
NADH | 25°C, wild-type enzyme | Homo sapiens | |
0.088 | - |
NADH | 25°C, mutant enzyme K337L | Homo sapiens | |
0.089 | - |
NADH | 25°C, mutant enzyme G446D | Homo sapiens | |
0.09 | - |
NADH | 25°C, mutant enzyme K344L | Homo sapiens | |
0.098 | - |
NADH | 25°C, mutant enzyme S445L | Homo sapiens | |
0.122 | - |
NADH | 25°C, mutant enzyme H454Y | Homo sapiens | |
1.25 | - |
2-oxoglutarate | 25°C, wild-type enzyme | Homo sapiens | |
1.29 | - |
2-oxoglutarate | 25°C, mutant enzyme K333L | Homo sapiens | |
1.31 | - |
2-oxoglutarate | 25°C, mutant enzyme K344L | Homo sapiens | |
1.31 | - |
2-oxoglutarate | 25°C, mutant enzyme K346L | Homo sapiens | |
1.45 | - |
2-oxoglutarate | 25°C, mutant enzyme K337L | Homo sapiens | |
1.47 | - |
2-oxoglutarate | 25°C, mutant enzyme S445L | Homo sapiens | |
1.5 | - |
2-oxoglutarate | 25°C, mutant enzyme G446D | Homo sapiens | |
3.13 | - |
2-oxoglutarate | 25°C, mutant enzyme H454Y | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
6 * 56000, mutant enzyme H454Y | Homo sapiens |
56000 | - |
6 * 56000, wild-type enzyme and mutant enzymes K333L, K337L, K344L, K346L, S445L and G446L | Homo sapiens |
160000 | - |
mutant enzyme H454Y, gel filtration | Homo sapiens |
330000 | - |
wild-type enzyme and mutant enzymes K333L, K337L, K344L, K346L, S445L and G446L, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Homo sapiens | L-glutamate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 56000, wild-type enzyme and mutant enzymes K333L, K337L, K344L, K346L, S445L and G446L | Homo sapiens |
trimer | 6 * 56000, mutant enzyme H454Y | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93 | - |
NADH | 25°C, mutant enzyme K333L | Homo sapiens | |
95 | - |
NADH | 25°C, mutant enzyme H454Y | Homo sapiens | |
98 | - |
NADH | 25°C, mutant enzyme K337L | Homo sapiens | |
100 | - |
NADH | 25°C, mutant enzyme K346L | Homo sapiens | |
102 | - |
NADH | 25°C, mutant enzyme G446D | Homo sapiens | |
104 | - |
NADH | 25°C, wild-type enzyme | Homo sapiens | |
105 | - |
NADH | 25°C, mutant enzyme K344L | Homo sapiens | |
111 | - |
NADH | 25°C, mutant enzyme S445L | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |