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Literature summary for 1.4.1.3 extracted from

  • DiRuggiero, J.; Robb, F.T.; Jagus, R.; Klump, H.H.; Borges, K.M.; Kessel, M.; Mai, X.; Adams, M.W.W.
    Characterization, cloning, and in vitro expression of the extremely thermostable glutamate dehydrogenase from the hyperthermophilic archaeon, ES4 (1993), J. Biol. Chem., 268, 17767-17774.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression by in vitro transcription/translation in rabbit reticulocytes Pyrococcus sp.

General Stability

General Stability Organism
absolutely stable at 4°C or at room temperature for at least 6 months Pyrococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
6 * 46000, SDS-PAGE Pyrococcus sp.
270000
-
gel filtration Pyrococcus sp.

Organism

Organism UniProt Comment Textmining
Pyrococcus sp.
-
ES4, hyperthermophilic archeon
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Pyrococcus sp. 2-oxoglutarate + NH3 + NAD(P)H
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 46000, SDS-PAGE Pyrococcus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100 105 1 mg/ml enzyme, half-life of 10.5 h at 100°C, 3.5 h at 105°C, and 20 h at 90°C, thermal denaturation at 113°C Pyrococcus sp.

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for Pyrococcus sp.