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Literature summary for 1.4.1.3 extracted from

  • Lee, A.R.; Balinsky, J.B.
    A kinetic study of glutamate dehydrogenase from xenopus laevis (1974), Int. J. Biochem., 5, 795-805.
No PubMed abstract available
Search for more literature for 1.4.1.3

Activating Compound

Activating Compound Comment Organism Structure
ADP stimulation Xenopus laevis

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate
-
 Xenopus laevis
NAD+
-
 Xenopus laevis
NADH high concentration Xenopus laevis
NH4Cl
-
 Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADH liver enzyme Xenopus laevis
0.022
-
 NADH kidney enzyme Xenopus laevis
0.032
-
 NAD+ kidney enzyme Xenopus laevis
0.4
-
 L-glutamate kidney enzyme Xenopus laevis
1
-
 2-oxoglutarate liver enzyme Xenopus laevis
2.3
-
 2-oxoglutarate kidney enzyme Xenopus laevis
20
-
 NH3 kidney enzyme Xenopus laevis
21
-
 NH3 liver enzyme Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Xenopus laevis
-
liver
-
 Xenopus laevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+
-
 Xenopus laevis L-glutamate + H2O + NAD+
-
 r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Xenopus laevis L-glutamate + H2O + NAD+
-
 r
L-glutamate + H2O + NAD(P)+
-
 Xenopus laevis 2-oxoglutarate + NH3 + NAD(P)H
-
 r
L-glutamate + H2O + NAD+
-
 Xenopus laevis 2-oxoglutarate + NH3 + NADH + H+
-
 r

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.011
-
 NADH liver enzyme Xenopus laevis
0.016
-
 NADH kidney enzyme Xenopus laevis
0.3
-
 NAD+ kidney enzyme Xenopus laevis
2.6
-
 2-oxoglutarate liver enzyme Xenopus laevis
5
-
 2-oxoglutarate kidney enzyme Xenopus laevis
46
-
 NH4Cl liver enzyme Xenopus laevis
69
-
 NH4Cl kidney enzyme Xenopus laevis