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Literature summary for 1.4.1.3 extracted from
- Characterization of an extremely thermostable glutamate dehydrogenase: a key enzyme in the primary metabolism of the hyperthermophilic archaebacterium, Pyrococcus furiosus (1992), Biochim. Biophys. Acta, 1120, 267-272.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
NADP+ | substrate L-glutamate | Pyrococcus furiosus |  |
| 0.53 | - |
NAD+ | substrate L-glutamate | Pyrococcus furiosus | |
| 0.56 | - |
NADPH | substrate 2-oxoglutarate | Pyrococcus furiosus | |
| 0.56 | - |
NADPH | substrate NH3 | Pyrococcus furiosus | |
| 0.98 | - |
NADH | substrate 2-oxoglutarate | Pyrococcus furiosus | |
| 1.6 | - |
L-glutamate | cofactor NADP+ | Pyrococcus furiosus | |
| 1.66 | - |
L-glutamate | cofactor NAD+ | Pyrococcus furiosus | |
| 4 | - |
2-oxoglutarate | cofactor NADPH | Pyrococcus furiosus | |
| 4.5 | - |
2-oxoglutarate | cofactor NADH | Pyrococcus furiosus | |
| 9.5 | - |
NH3 | cofactor NADPH | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
6 * 46000, SDS-PAGE | Pyrococcus furiosus |
| 270000 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 32.5 | - |
at 85°C | Pyrococcus furiosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 6 months, stable | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD(P)+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 46000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
half-life: 2.3 h at 0.053 mg/ml protein concentration, 10 h at 1.06 mg/ml protein concentration | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | enzyme activity with NADP+ is higher than the activity with NAD+ | Pyrococcus furiosus | |
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