General Stability | Organism |
---|---|
SDS: 0.1% v/v, no loss of activity after 12 h, 0.5%, half-life 5 h | Saccharolobus solfataricus |
urea: 4 M, 10% loss of activity after 12 h, 7.5 M, half-life 9 h | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glutamate | 2 mM, 11% inhibition, 6 mM, 34% inhibition | Saccharolobus solfataricus | |
pyridoxal 5'-phosphate | 2 mM, complete loss of activity | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
NADH | + 2-oxoglutarate | Saccharolobus solfataricus | |
0.01 | - |
NADPH | + 2-oxoglutarate | Saccharolobus solfataricus | |
0.025 | - |
NADP+ | + L-glutamate | Saccharolobus solfataricus | |
0.063 | - |
NAD+ | + L-glutamate | Saccharolobus solfataricus | |
0.2 | - |
2-oxoglutarate | cosubstrate NADPH | Saccharolobus solfataricus | |
0.3 | - |
L-glutamate | cosubstrate NAD+ | Saccharolobus solfataricus | |
0.6 | - |
2-oxoglutarate | cosubstrate NADH | Saccharolobus solfataricus | |
1.1 | - |
L-glutamate | cosubstrate NADP+ | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
6 * 44000, SDS-PAGE | Saccharolobus solfataricus |
270000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
isopropanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
Methanol | 50% v/v, complete loss of activity | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
423 | - |
at 70°C | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-70°C, stable for 6 months | Saccharolobus solfataricus |
4°C, 20% loss of activity after 40 days | Saccharolobus solfataricus |
4°C, more than 20 days, no loss of activity | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Saccharolobus solfataricus | L-glutamate + H2O + NAD+ | - |
? | |
L-glutamate + H2O + NAD(P)+ | highly specific for 2-oxoglutarate and glutamate | Saccharolobus solfataricus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 44000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | 30°C: about 90% of activity maximum, 90°C: about 35% of activity maximum | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | no loss in activity after 1 h at 90°C, 20% residual activity after 1 h at 100°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
NAD(P)H + 2-oxoglutarate + NH3 | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 90-100% activity | Saccharolobus solfataricus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | at least 65% residual activity after 14 h | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Saccharolobus solfataricus | |
NADPH | - |
Saccharolobus solfataricus |