General Stability | Organism |
---|---|
freeze-thawing decreases activity | Bacillus subtilis |
glycerol, 5-20% v/v, stabilizes | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 10 mM, 60% inhibition of oxidative deamination | Bacillus subtilis | |
oxaloacetate | 5 mM, 79% inhibition of oxidative deamination | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Bacillus subtilis |
270000 | - |
gel filtration, sucrose density gradient centrifugation | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis PCI 219 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Bacillus subtilis |
Storage Stability | Organism |
---|---|
0°C, 50 mM Na,K phosphate buffer, pH 5.5, stable | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Bacillus subtilis | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD(P)+ | - |
Bacillus subtilis PCI 219 | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
10 min, 50% loss of activity | Bacillus subtilis |
74 | - |
10 min, complete loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
NADPH + NH3 + 2-oxoglutarate | Bacillus subtilis |
7.6 | - |
NADP+ + glutamate | Bacillus subtilis |
7.8 | - |
NADH + NH3 + 2-oxoglutarate | Bacillus subtilis |
8.2 | - |
NAD+ + H2O + glutamate | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | NADPH/NADH ratio of reaction rate: 10/1, NAD+/NADH ratio of reaction rate: 5/1 | Bacillus subtilis |