Cloned (Comment) | Organism |
---|---|
gene msmeg_4699, semi-quantitative real-time PCR expression analysis | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glycogen accumulation regulator | GarA, native or unphosphorylated GarA is able to interact with NAD+-GDH causing a reduction in NAD+-GDH activity by altering the affinity of the enzyme for its substrate. This binding is prevented by the phosphorylation of GarA by PknG | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Mycolicibacterium smegmatis | reaction cycle, specificities of forward and reverse reactions, overview | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R1C2 | gene msmeg_4699 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | reaction cycle, specificities of forward and reverse reactions, overview | Mycolicibacterium smegmatis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NAD+ | reaction cycle, overview | Mycolicibacterium smegmatis | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH2 | - |
Mycolicibacterium smegmatis |
More | NAD+-specific glutamate dehydrogenases belongs to the L_180 class, that is affected by the binding of a small protein, GarA | Mycolicibacterium smegmatis |
NAD+-dependant glutamate dehydrogenase | - |
Mycolicibacterium smegmatis |
NAD+-GDH | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for | Mycolicibacterium smegmatis | |
NADH | specific for | Mycolicibacterium smegmatis |
Organism | Comment | Expression |
---|---|---|
Mycolicibacterium smegmatis | enzyme expression is upregulated under N2-starvation, overview | up |
General Information | Comment | Organism |
---|---|---|
metabolism | NAD+-GDH plays an important role in nitrogen assimilation rather than glutamate catabolism, and is involved in the additional nitrogen assimilatory pathway via glutamate dehydrogenase, GDH. The specific activity of the deaminating NAD+-GDH reaction is mostly independent of nitrogen availability, overview, overview | Mycolicibacterium smegmatis |