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Literature summary for 1.4.1.2 extracted from

  • Jahns, T.; Kaltwasser, H.
    Properties of the cold-labile NAD+-specific glutamate dehydrogenase from Bacillus cereus DSM 31 (1993), J. Gen. Microbiol., 139, 775-780.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP Bacillus cereus

General Stability

General Stability Organism
extremely unstable at 0°C to 4°C due to the dissociation of the holoenzyme into catalytically inactive subunits Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
NH4+ for 200 mM NH4+ Bacillus cereus
0.56
-
NAD+
-
Bacillus cereus
7.4
-
glutamate
-
Bacillus cereus
96
-
NH4+ from double-reciprocal plots for concentrations between 5 mM and 200 mM Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
gel filtration Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
strain DSM 31
-

Purification (Commentary)

Purification (Comment) Organism
18-22°C, 20% glycerol, heat treatment, ammonium sulfate, gel filtration, ion-exchange chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
286.4
-
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Bacillus cereus L-glutamate + NADP+
-
?
L-glutamate + H2O + NAD(P)+
-
Bacillus cereus 2-oxoglutarate + NH3 + NAD(P)H
-
r
L-glutamate + H2O + NAD+
-
Bacillus cereus 2-oxoglutarate + NH3 + NADH + H+
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-
-
-
Bacillus cereus
20 25 no activity in cell extracts prepared at 0 to 4°C, high activities between 20°C and 25°C, 70% activity is retained after 2 h at 0°C in the presence of 20% glycerol Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
reductive amination of 2-oxoglutarate Bacillus cereus
9.4
-
oxidative deamination of glutamate Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+ no activity with NADP+ Bacillus cereus