Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP | Bacillus cereus |
General Stability | Organism |
---|---|
extremely unstable at 0°C to 4°C due to the dissociation of the holoenzyme into catalytically inactive subunits | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition or activation in the presence of 500 mM AMP, ADP, ATP, cyclic-AMP, GMP, GDP or GTP | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
NH4+ | for 200 mM NH4+ | Bacillus cereus | |
0.56 | - |
NAD+ | - |
Bacillus cereus | |
7.4 | - |
glutamate | - |
Bacillus cereus | |
96 | - |
NH4+ | from double-reciprocal plots for concentrations between 5 mM and 200 mM | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
gel filtration | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
strain DSM 31 | - |
Purification (Comment) | Organism |
---|---|
18-22°C, 20% glycerol, heat treatment, ammonium sulfate, gel filtration, ion-exchange chromatography | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
286.4 | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Bacillus cereus | L-glutamate + NADP+ | - |
? | |
L-glutamate + H2O + NAD(P)+ | - |
Bacillus cereus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r | |
L-glutamate + H2O + NAD+ | - |
Bacillus cereus | 2-oxoglutarate + NH3 + NADH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
- |
Bacillus cereus |
20 | 25 | no activity in cell extracts prepared at 0 to 4°C, high activities between 20°C and 25°C, 70% activity is retained after 2 h at 0°C in the presence of 20% glycerol | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
reductive amination of 2-oxoglutarate | Bacillus cereus |
9.4 | - |
oxidative deamination of glutamate | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | no activity with NADP+ | Bacillus cereus |