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Literature summary for 1.4.1.2 extracted from
- Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents (1998), Appl. Environ. Microbiol., 64, 2152-2157.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetonitrile | activates | Pyrobaculum islandicum |  |
| guanidine hydrochloride | activates | Pyrobaculum islandicum | |
| tetrahydrofuran | activates | Pyrobaculum islandicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NADH | - |
Pyrobaculum islandicum | |
| 0.025 | - |
NAD+ | - |
Pyrobaculum islandicum | |
| 0.066 | - |
2-oxoglutarate | - |
Pyrobaculum islandicum | |
| 0.17 | - |
L-glutamate | - |
Pyrobaculum islandicum | |
| 9.7 | - |
NH4+ | - |
Pyrobaculum islandicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
6 * 36000, SDS-PAGE | Pyrobaculum islandicum |
| 220000 | - |
gel filtration | Pyrobaculum islandicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrobaculum islandicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrobaculum islandicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Pyrobaculum islandicum | L-glutamate + NADP+ | - |
? | |
| L-glutamate + H2O + NAD+ | - |
Pyrobaculum islandicum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 36000, SDS-PAGE | Pyrobaculum islandicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrobaculum islandicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
no loss of activity after 2 h | Pyrobaculum islandicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pyrobaculum islandicum | |
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Release 2023.1 (January 2023)
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