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Literature summary for 1.3.99.5 extracted from
- Cloning and differential expression of steroid 5alpha-reductase type 1 (Srd5a1) and type 2 (Srd5a2) from the Harderian glands of hamsters (2010), Gen. Comp. Endocrinol., 166, 388-395.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Srd5a1 DNA and amino acid sequence determination and analysis, expression of Srd5a2 in HEK-293 cells, real-time quantitative RT-PCR expression analysis with higher mRNA levels for Srd5a1 than for Srd5a2 | Mesocricetus auratus |
| Srd5a2 DNA and amino acid sequence determination and analysis, expression of Srd5a2 in HEK-293 cells, real-time quantitative RT-PCR expression analysis with higher mRNA levels for Srd5a1 than for Srd5a2 | Mesocricetus auratus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0019 | - |
testosterone | Srd5a2, pH not specified in the publication, temperature not specified in the publication | Mesocricetus auratus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrotestosterone + NADP+ | Mesocricetus auratus | the isoenzyme efficiently catalyzes the in vitro transformation of testosterone into dihydrotestosterone | testosterone + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | C7T2J7 | Srd5a1 | - |
| Mesocricetus auratus | C7T2J8 | Srd5a2 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epididymis | Srd5a2 | Mesocricetus auratus | - |
| harderian gland | both Srd5a1 and Srd5a2 are expressed without clear differences between males and females | Mesocricetus auratus | - |
| liver | Srd5a1 | Mesocricetus auratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrotestosterone + NADP+ | - |
Mesocricetus auratus | testosterone + NADPH + H+ | - |
r | |
| dihydrotestosterone + NADP+ | the isoenzyme efficiently catalyzes the in vitro transformation of testosterone into dihydrotestosterone | Mesocricetus auratus | testosterone + NADPH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SRD5A1 | - |
Mesocricetus auratus |
| SRD5A2 | - |
Mesocricetus auratus |
| steroid 5alpha-reductase type 1 | - |
Mesocricetus auratus |
| steroid 5alpha-reductase type 2 | - |
Mesocricetus auratus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant Srd5a2 | Mesocricetus auratus |
| 6.5 | 8 | recombinant Srd5a1 | Mesocricetus auratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Mesocricetus auratus | |
| NADPH | - |
Mesocricetus auratus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mesocricetus auratus | 17beta-estradiol administered to spayed females induces the up-regulation of Srd5a2 mRNA levels | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the isozyme may play a relevant role in modulating androgen signaling in Hardarian glands | Mesocricetus auratus |
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